Involvement of Aif1 in apoptosis triggered by lack of Hxk2 in the yeast Saccharomyces cerevisiae
| Autor: | Gianluca Frigerio, Loredana Amigoni, Sonia Colombo, Enzo Martegani |
|---|---|
| Přispěvatelé: | Amigoni, L, Frigerio, G, Martegani, E, Colombo, S |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Regulated cell death Apoptosis Biology Mitochondrion Acetic acid Applied Microbiology and Biotechnology Microbiology Membrane Potentials Cell membrane 03 medical and health sciences chemistry.chemical_compound HXK2 Hexokinase medicine NADH NADPH Oxidoreductases chemistry.chemical_classification Reactive oxygen species General Medicine biology.organism_classification AIF1 Hydrogen peroxide BIO/11 - BIOLOGIA MOLECOLARE Molecular biology Yeast Cell biology Cell nucleus 030104 developmental biology medicine.anatomical_structure chemistry Mitochondrial Membranes Reactive Oxygen Species Gene Deletion |
| Popis: | We recently showed that in hxk2 Δ cells, showing constitutive localization of active Ras at the mitochondria, addition of acetic acid caused an increase of both apoptotic and necrotic cells compared with the wild-type strain, providing a new role for hexokinase 2 (EC 2.7.1.1) as an anti-apoptotic factor, besides its known role as a glycolytic enzyme and as a regulator of gene transcription of several Mig1‐regulated genes. We also demonstrated that apoptosis induced by lack of Hxk2 may not require the activation of Yca1. Here, we show that deletion of HXK2 causes hypersensitivity to H2O2 and that addition of this well-known apoptotic stimulus to hxk2 Δ cells causes an increase in the level ROS, apoptosis and mitochondrial membrane potential. We also show that deletion of AIF1 in hxk2 Δ cells enhances survival after induction of apoptosis with both H2O2 and acetic acid, rescues the reduction of both growth rate and cell size, abrogates both H2O2 and acetic acid-induced ROS accumulation and decreases cell death, suggesting that Aif1 might be involved in both H2O2 and acetic acid‐induced cell death in hxk2 Δ cells. Moreover, we show that active Ras proteins relocalize to the plasma membrane and to the nucleus in hxk2 Δ aif1 Δ cells. |
| Databáze: | OpenAIRE |
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