Assessing Almond and Peanut Allergens Using Commercially Available Immunoanalytical Kits and LC-MS/MS: A Case Study
| Autor: | Georg Häubl, Parisa Ansari, Kurt Brunner, Adrian Rogers, Matthew Daly |
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| Rok vydání: | 2018 |
| Předmět: |
Arachis
Population Enzyme-Linked Immunosorbent Assay Tandem mass spectrometry 01 natural sciences Analytical Chemistry Elisa kit 0404 agricultural biotechnology Tandem Mass Spectrometry Lc ms ms Humans Environmental Chemistry Prunus dulcis/chemistry Food science education Pharmacology High concentration education.field_of_study Chemistry 010401 analytical chemistry food and beverages Chromatography liquid Arachis/chemistry 04 agricultural and veterinary sciences Allergens Prunus dulcis 040401 food science Food hypersensitivity 0104 chemical sciences Allergens/analysis Agronomy and Crop Science Food Hypersensitivity Chromatography Liquid Food Science |
| Zdroj: | Daly, M, Ansari, P, Häubl, G, Rogers, A & Brunner, K 2018, ' Assessing Almond and Peanut Allergens Using Commercially Available Immunoanalytical Kits and LC-MS/MS : A Case Study ', Journal of AOAC INTERNATIONAL, vol. 101, no. 1, pp. 96-101 . https://doi.org/10.5740/jaoacint.17-0398 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.17-0398 |
| Popis: | With an ever-increasing allergic population and an emerging market for allergen-free foods, accurate detection of allergens in foods has never been more important. Although ELISA-based methods are the most widely used for detection of allergens in food, there is a need for the development of orthogonal approaches. A commercial ELISA detected a relatively high concentration of peanut and almond in an allergen-free product. However, another commercial ELISA declared a low peanut concentration and was negative for almond. Further testing using a commercial almond lateral-flow device confirmed the results from the second ELISA kit and demonstrated that the positive detection of almond was due to cross-reactivity. An MS method was used for final confirmation that the reported results were negative for both almond and peanut. |
| Databáze: | OpenAIRE |
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