Amyloidogenic processing but not amyloid precursor protein (APP) intracellular C-terminal domain production requires a precisely oriented APP dimer assembled by transmembrane GXXXG motifs
| Autor: | Jeffrey Z. Fei, Saburo Aimoto, Mingli Li, Pascal Kienlen-Campard, Stefan N. Constantinescu, Joanne Van Hees, Jean-Noël Octave, Bernadette Tasiaux, Pierre J. Courtoy, Sandra Huysseune, Takeshi Sato, Steven O. Smith |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Transcription
Genetic Mutant Amino Acid Motifs Peptide CHO Cells Biochemistry Article Amyloid beta-Protein Precursor Protein structure Cricetulus Cricetinae mental disorders Amyloid precursor protein Animals Humans Senile plaques Molecular Biology chemistry.chemical_classification biology Chemistry C-terminus Cell Membrane Cell Biology Transmembrane protein Cell biology Protein Structure Tertiary Mutation biology.protein Amyloid Precursor Protein Secretases Amyloid precursor protein secretase Dimerization |
| Zdroj: | The Journal of biological chemistry. 283(12) |
| ISSN: | 0021-9258 |
| Popis: | The β-amyloid peptide (Aβ) is the major constituent of the amyloid core of senile plaques found in the brain of patients with Alzheimer's disease (AD). Aβ is produced by the sequential cleavage of the Amyloid Precursor Protein (APP) by β- and γ-secretases. Cleavage of APP by γ-secretase also generates the APP Intracellular C-terminal Domain (AICD) peptide, which might be involved in regulation of gene transcription. APP contains three glycine-xxx-glycine (GxxxG) motifs in its juxtamembrane and transmembrane (TM) regions. Such motifs are known to promote dimerization via close apposition of TM sequences. We demonstrate that pairwise replacement of glycines by leucines or isoleucines, but not alanines, in a GxxxG motif led to a drastic reduction of Aβ40 and Aβ42 secretion. β-Cleavage of mutant APP was not inhibited, and reduction of Aβ secretion resulted from inhibition of γ-cleavage. It was anticipated that decreased γ-cleavage of mutant APP would result from inhibition of its dimerization. Surprisingly, mutations of the GxxxG motif actually enhanced dimerization of the APP C-terminal fragments, possibly via a different TM α-helical interface. Increased dimerization of the TM APP C-terminal domain did not affect AICD production. These results clearly demonstrate that both orientation and dimerization of the APP TM domain differently affect Aβ and AICD production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|