Metabolite Profiling of Soybean Seed Extracts from Near-Isogenic Low and Normal Phytate Lines Using Orthogonal Separation Strategies
| Autor: | Elizabeth A. Grabau, Sherry B. Hildreth, Richard F. Helm, Christin Kastl, Judith Jervis, R. M. Biyashev, Mohammad A. Saghai-Maroof |
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| Rok vydání: | 2015 |
| Předmět: |
Phytic Acid
Arginine Metabolite Mutant Oligosaccharides Tandem mass spectrometry chemistry.chemical_compound Metabolomics Tandem Mass Spectrometry Plant Proteins chemistry.chemical_classification Chromatography Plant Extracts Wild type General Chemistry Saponins Isoflavones Amino acid chemistry Biochemistry Mutation Seeds Soybeans General Agricultural and Biological Sciences Chromatography Liquid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 63:9879-9887 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS) was applied to lipid-depleted methanolic extracts of soybean seeds utilizing orthogonal chromatographic separations (reversed-phase and hydrophilic interaction) in both positive and negative ionization modes. Four near-isogenic lines (NILs) differing in mutations for two genes encoding highly homologous multidrug resistant proteins (MRPs) were evaluated. The double mutant exhibited a low phytate phenotype, whereas the other three NILs, the two single mutants and the wild type, did not. Principal component analysis (PCA) of the four LC-MS data sets fully separated the low phytate line from the other three. While the levels of neutral oligosaccharides were the same for all lines, there were significant metabolite differences residing in the levels of malonyl isoflavones, soyasaponins, and arginine. Two methanol-soluble polypeptides were also found as differing in abundance levels, one of which was identified as the allergen Gly m 1. |
| Databáze: | OpenAIRE |
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