Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester
| Autor: | J Y Richmond, R C Knudsen, J Polatnick |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Microculture
Viral Plaque Assay Vesicular stomatitis Indiana virus Biology Applied Microbiology and Biotechnology Cell Line Mice chemistry.chemical_compound Interferon medicine Animals Uridine Virus quantification Ecology biology.organism_classification Virology Molecular biology Titer chemistry Cell culture Vesicular stomatitis virus RNA Viral Biological Assay Interferons Research Article Food Science Biotechnology medicine.drug |
| Zdroj: | Applied and Environmental Microbiology. 39:823-827 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.39.4.823-827.1980 |
| Popis: | A microassay for interferon is described which uses target cells grown in microculture wells, [3H]uridine to measure vesicular stomatitis virus replication in target cells, and a multiple automated sample harvester to collect the radioactively labeled viral ribonucleic acid onto glass fiber filter disks. The disks were placed in minivials, and radioactivity was counted in a liquid scintillation spectrophotometer. Interferon activity was calculated as the reciprocal of the highest titer which inhibited the incorporation of [3H]uridine into viral ribonucleic acid by 50%. Interferon titers determined by the microassay were similar to the plaque reduction assay when 100 plaque-forming units of challenge vesicular stomatitis virus was used. However, it was found that the interferon titers decreased approximately 2-fold for each 10-fold increase in the concentration of challenge vesicular stomatitis virus when tested in the range of 10(2) to 10(5) plaque-forming units. Interferon titers determined by the microassay show a high degree of repeatability, and the assay can be used to measure small and large numbers of interferon samples. |
| Databáze: | OpenAIRE |
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