Live pups from evaporatively dried mouse sperm stored at ambient temperature for up to 2 years

Autor: Mehmet Toner, John D. Biggers, Jie Liu, Joel A. Lawitts, Gloria Y. Lee
Rok vydání: 2014
Předmět:
Male
Embryology
Time Factors
lcsh:Medicine
Mice
0302 clinical medicine
Human fertilization
Pregnancy
lcsh:Science
reproductive and urinary physiology
Cells
Cultured
0303 health sciences
Mice
Inbred ICR
030219 obstetrics & reproductive medicine
Multidisciplinary
Temperature
Embryo
Animal Models
Embryo transfer
medicine.anatomical_structure
Engineering and Technology
Female
Live Birth
Research Article
Biotechnology
Cryobiology
Offspring
Biomedical Engineering
Bioengineering
Mouse Models
Biology
Research and Analysis Methods
Sperm Preservation
Andrology
03 medical and health sciences
Model Organisms
medicine
Animals
Blastocyst
Sperm Injections
Intracytoplasmic
Desiccation
030304 developmental biology
urogenital system
lcsh:R
Biology and Life Sciences
Embryo Transfer
Sperm
Mice
Inbred C57BL
Animals
Newborn
Immunology
lcsh:Q
Semen Preservation
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
ISSN: 1932-6203
Popis: The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples stored at 4°C for 3, 6, 12, 18, and 24 months, the blastocyst formation rate was 61.5%, 49.1%, 31.5%, 32.2%, and 41.4%, respectively. The blastocyst rate for those stored at ambient temperature (∼22°C) for 3, 6, 12, and 18 months was 57.8%, 36.2%, 33.6%, and 34.4%, respectively. Fifteen, eight and three live pups were produced from sperm stored at room temperature for 12, 18, and 24 months, respectively. This is the first report of live offspring produced from dried mouse sperm stored at ambient temperature for up to 2 years. Based on these results, we suggest that evaporative drying is a potentially useful method for the routine preservation of mouse sperm.
Databáze: OpenAIRE
Externí odkaz: