Alterations in [3H]MK-801, [3H]muscimol, [3H]cyclic AMP, and [3H]rolipram binding in the gerbil hippocampus following repeated ischemic insults
Autor: | Kenshi Murase, Kyuya Kogure, Hiroyuki Kato, Tsutomu Araki |
---|---|
Rok vydání: | 1993 |
Předmět: |
Male
medicine.medical_specialty Phosphodiesterase Inhibitors Ischemia Gerbil Hippocampus Brain Ischemia chemistry.chemical_compound Internal medicine Cyclic AMP medicine Animals Rolipram Histocytochemistry Muscimol Chemistry GABAA receptor General Neuroscience Glutamate receptor Phosphodiesterase medicine.disease Pyrrolidinones Endocrinology nervous system 3' 5'-Cyclic-AMP Phosphodiesterases Second messenger system Autoradiography Dizocilpine Maleate Gerbillinae Protein Kinases medicine.drug |
Zdroj: | Neuroscience. 52:245-253 |
ISSN: | 0306-4522 |
DOI: | 10.1016/0306-4522(93)90153-7 |
Popis: | Using [ 3 H]MK-801, [ 3 H]muscimol, [ 3 H]cyclic AMP, and [ 3 H]rolipram, we performed quantitative in vitro autoradiography to determine sequential alterations in the binding of N -methyl- d -aspartate and GABA A receptors, particulate cyclic AMP-dependent protein kinase, and cyclic AMP-selective phosphodiesterase, respectively, in the gerbil hippocampus following repeated brief ischemic insults. Changes from 1 h to 28 days after three 2-min ischemic insults at 1-h intervals were compared with those after 2 and 6 min of ischemia. We observed no alterations in the binding of all the four ligands throughout the observation period following 2 min of ischemia which produced no histological neuronal damage except for transient reductions in [ 3 H]cyclic AMP binding during the early reperfusion period. [ 3 H]Cyclic AMP binding in the CA1 subfield decreased one day after 6 min of ischemia and four days after three 2-min ischemic insults, and 62–65% of the binding was lost after 28 days. [ 3 H]Rolipram binding in the CA1 subfield decreased one day after 6 min of ischemia and the binding was reduced by 45–50% after four and 28 days. Following three 2-min ischemic insults, [ 3 H]rolipram binding decreased in the CA1 at one day, and decreased by 25–33% after 28 days. Both [ 3 H]MK-801 and [ 3 H]muscimol binding was preserved during the early reperfusion period after 6 min of ischemia and three 2-min ischemic insults. Reductions in [ 3 H]MK-801 binding in CA1 were observed four days after ischemic insults when CA1 neuronal destruction was seen. After one month, approximately 50% of [ 3 H]MK-801 binding was lost in CA1 in both groups. By contrast, reductions in [ 3 H]muscimol binding (by 20–30%) were only observed at one month. Thus, alterations in the cyclic AMP-related second messenger system preceded histological neuronal death of CA1 pyramidal cells. The results also suggest that N -methyl- d -aspartate receptors play a role in ischemia-induced CA1 neuronal damage. Furthermore, we observed a different pattern of injury following repeated versus single ischemic episodes. Differential localization of N -methyl- d -asparate and GABA A receptors, and of cyclic AMP-dependent protein kinase and phosphodiesterase within different subrogions of the hippocampus was also suggested. |
Databáze: | OpenAIRE |
Externí odkaz: |