Replication of Equine Herpesvirus Type 1 and Type 3: Resistance to Hydroxyurea and Thymidine
| Autor: | James C. Cohen, George P. Allen, Charles C. Randall, Dennis J. O'Callaghan |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Cell
Centrifugation Isopycnic Biology Virus Replication chemistry.chemical_compound Virology medicine Animals Hydroxyurea Horses Cells Cultured Herpesviridae Differential centrifugation DNA synthesis DNA replication Drug Resistance Microbial Molecular biology Infectious Diseases medicine.anatomical_structure Ribonucleotide reductase chemistry DNA Viral Equine herpesvirus Thymidine DNA |
| Zdroj: | Intervirology. 9:276-285 |
| ISSN: | 1423-0100 0300-5526 |
| DOI: | 10.1159/000148945 |
| Popis: | The replication of equine herpesvirus type 1 (EHV-1) and type 3 (EHV-3) was unimpeded in three different cell types – equine epithelial cells, equine fibroblasts, and mouse fibroblasts – which had been blocked in their capacity to synthesize host DNA by 2.5 mM hydroxyurea (HU) or 2 mM thymidine (TdR). The rate of DNA synthesis in un-infected or equine herpesvirus-infected cells in the presence of HU or TdR was measured by pulse-labeling cell samples with a labeled DNA precursor at different times after infection. DNA synthesis in uninfected cultures was completely inhibited by both compounds. However, in cells infected with EHV-1 or EHV-3 and incubated in the presence of HU or TdR, a burst of DNA synthesis occurred which coincided in time with that of virus-specific DNA replication in infected cells without inhibitors. Analysis by cesium chloride isopycnic centrifugation confirmed that the DNA made in drug-treated, infected cells was viral. A possible mechanism of the HU and TdR resistance of the equine herpesviruses is the induction of a ribonucleotide reductase which is insensitive to inhibition by these compounds. |
| Databáze: | OpenAIRE |
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