Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
| Autor: | Daniel McCluskey, Ian Munro, Loïc Coudron, C. K. L. Tan, Martin B. McDonnell, Nikolay Dimov, I. D. Johnston |
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| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
Enzyme-Linked Immunosorbent Assay 02 engineering and technology 01 natural sciences General Biochemistry Genetics and Molecular Biology Luminol law.invention Contact angle Automation chemistry.chemical_compound law Lab-On-A-Chip Devices 0202 electrical engineering electronic engineering information engineering Surface charge Digital microfluidics Antigens Chemiluminescence Chromatography General Immunology and Microbiology biology General Neuroscience 010401 analytical chemistry Charge density 020206 networking & telecommunications Primary and secondary antibodies 0104 chemical sciences Electrowetting chemistry biology.protein Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/60489 |
| Popis: | Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H2O2/Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized. |
| Databáze: | OpenAIRE |
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