Expression of heme oxygenase-1 in the lungs of rats exposed to potassium octatitanate whiskers
| Autor: | Hiroko Nagatomo, Takako Oyabu, Masami Hirohashi, Yasuo Morimoto, Seichi Horie, Akira Ogami, Toshihiko Myojo, Yasuko Obata, Masahiro Murakami, Isamu Tanaka, Shoko Kawanami |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Pathology Gene Expression Lung injury medicine.disease_cause chemistry.chemical_compound Internal medicine Pulmonary fibrosis Gene expression Macrophages Alveolar medicine Animals RNA Messenger Rats Wistar Heme Lung Mineral Fibers Titanium Public Health Environmental and Occupational Health Epithelial Cells medicine.disease Rats Heme oxygenase Oxidative Stress Endocrinology medicine.anatomical_structure chemistry Immunostaining Oxidative stress Heme Oxygenase-1 |
| Zdroj: | Journal of occupational health. 53(4) |
| ISSN: | 1348-9585 |
| Popis: | Objectives: Oxidative stress is thought to be the pathogenesis of pulmonary fibrosis induced by asbestos, and heme oxygenase-1 (HO-1) protects lung tissue against oxidative stress. We hypothesized that HO-1 is also associated with oxidative lung injury caused by exposure to potassium octatitanate whiskers (PT1), which is one of the asbestos substitutes. Methods: Male Wistar rats were administered 1 mg or 2 mg PT1 suspended in saline by a single intratracheal instillation and were sacrificed after recovery for 3 days, 1 wk, 1 mo, 3 mo or 6 mo. Gene expression of HO-1 protein and mRNA and immunostaining were investigated in rat lungs. Results: HO-1 protein expression was increased from 3 days to 1 mo and at 6 mo in the 1 or 2 mg PT1-exposed groups, and the gene expression of HO-1 mRNA was also increased at 3 days and from 1 mo to 6 mo. HO-1-positive cells were mainly found in the alveolar macrophages and the bronchial epithelial cells in immunostaining. Conclusions: These findings suggest that HO-1 is involved in lung damage caused by PT1. |
| Databáze: | OpenAIRE |
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