Proteomic characterization of lipid rafts markers from the rat intestinal brush border
| Autor: | Comeau-Druet Danielle, Valérie Deyris, Abdul A. Waheed, Muriel Rozière, Adda Berkane Amine, Daniel Lafitte, Abel Hiol, Hang Thi Thu Nguyen, Louis Comeau, Claude Villard, Marion Létisse, Cendrine Nicoletti, Léopold Tchiakpe |
|---|---|
| Přispěvatelé: | Physiologie neurovégétative - PNV (PNP), Université Paul Cézanne - Aix-Marseille 3-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Cytosquelette et Intégration des Signaux du Micro-Environnement Tumoral - UMR 6032 (CISMET), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Université de la Méditerranée - Aix-Marseille 2, Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Proteomics Brush border G protein Glycosylphosphatidylinositols [SDV]Life Sciences [q-bio] Detergents Galectin 4 Biophysics Biology GPI-Linked Proteins Biochemistry Aminopeptidase Membrane Microdomains Animals Rats Wistar Microscopy Immunoelectron Molecular Biology Lipid raft Annexin A2 Guanidine G protein-coupled receptor Ganglioside Membrane Glycoproteins Microvilli Phosphatidylinositol Diacylglycerol-Lyase Cell Biology Cell biology Rats Intestines Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Alkaline phosphatase lipids (amino acids peptides and proteins) Biomarkers |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2006, 342(1), pp.236-44. ⟨10.1016/j.bbrc.2006.01.141⟩ Biochemical and Biophysical Research Communications, 2006, 342(1), pp.236-44. ⟨10.1016/j.bbrc.2006.01.141⟩ |
| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/j.bbrc.2006.01.141⟩ |
| Popis: | To assess intestinal lipid rafts functions through the characterization of their protein markers, we have isolated lipid rafts of rat mucosa either from the total membrane or purified brush-border membrane (BBM) by sucrose gradient fractionation after detergent treatment. In both membrane preparations, the floating fractions (4-5) were enriched in cholesterol, ganglioside GM1, and N aminopeptidase (NAP) known as intestinal lipid rafts markers. Based on MALDI-TOF/MS identification and simultaneous detection by immunoblotting, 12 proteins from BBM cleared from contaminants were selected as rafts markers. These proteins include several signaling/trafficking proteins belonging to the G protein family and the annexins as well as GPI-anchored proteins. Remarkably GP2, previously described as the pancreatic granule GPI-anchored protein, was found in intestinal lipid rafts. The proteomic strategy assayed on the intestine leads to the characterization of known (NAP, alkaline phosphatase, dipeptidyl aminopeptidase, annexin II, and galectin-4) and new (GP2, annexin IV, XIIIb, Galpha(q), Galpha(11), glutamate receptor, and GPCR 7) lipid rafts markers. Together our results indicate that some digestive enzymes, trafficking and signaling proteins may be functionally distributed in the intestine lipid rafts. |
| Databáze: | OpenAIRE |
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