Staphylococcus aureus on the effect of expression of MMPs/TIMPs and uPA system in bovine mammary fibroblasts
| Autor: | Mengjuan Chen, Zengqiang Miao, Yannan Bi, Fenglong Wang, Xia Cao, Yulin Ding |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus Hot Temperature TIMPs 030106 microbiology Gene Expression Biology Matrix metalloproteinase medicine.disease_cause uPA system Microbiology Pathogenesis 03 medical and health sciences 0302 clinical medicine Western blot Fibrosis BMFBs medicine Animals Humans Immunology and Allergy 030212 general & internal medicine Mammary Glands Human chemistry.chemical_classification Messenger RNA Microbial Viability General Immunology and Microbiology medicine.diagnostic_test General Medicine Fibroblasts S. aureus medicine.disease Urokinase-Type Plasminogen Activator Molecular biology Matrix Metalloproteinases QR1-502 Urokinase receptor Infectious Diseases Enzyme chemistry Cattle Female MMPs |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 54, Iss 3, Pp 411-419 (2021) |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2019.09.008 |
| Popis: | Background Bovine mammary fibrosis is characteristic of chronic in injury in response to diverse pathogens. Staphylococcus aureus (S.aureus) is a frequent cause of mastitis in bovine and is prone to persistent infection. Diverse studies have shown MMPs/TIMPs and uPA system as a potent target for the treatment of fibrosis. However, pathogenesis of S. aureus-induced mammary fibrosis has not been completely defined. Methods BMFBs treated with heat-inactivated S. aureus (105, 106, and 108 CFU/mL) for 6, 12, 24, and 48 h. Total RNA and protein were isolated from the treatments and controls of BMFBs samples. MMP-1, MMP-2, MMP-3, MMP-9, MMP-13, TIMP-1, TIMP-2, COL Ⅰ, uPA, uPAR and PAI-1 gene and protein expression were examined by RT-qPCR and Western blot analysis. Gelatin zymography assay was performed to assess the levels of MMP-2 and MMP-9 enzyme secreted. Results BMFBs treated with heat-inactivated S. aureus increased mRNA and protein expression levels of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-13, and heat-inactivated S. aureus induced TIMP-1, TIMP-2 and COL Ⅰ expression. There was a clear activation of MMP-2 in the presence of heat-inactivated S. aureus in the conditioned medium from the BMFBs, whereas MMP-9 was no significantly altered. Moreover, uPA system was activated in BMFBs to S. aureus. Conclusion Activation of the uPA system together with its impact on the MMPs levels could play a significant role in S. aureus-induced BMFBs with mechanism of ECM metabolism, MMPs/TIMPs and uPA system could participate in bovine mammary fibrosis. |
| Databáze: | OpenAIRE |
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