| Popis: |
Figure S1. The sequencing figure shows the mutation patterns of the T0 transgenic lines 3 to 6. + 2, 2 bp insertion; − 10, 10 bp deletion; + 1, 1 bp insertion; − 3, 3 bp deletion. WT, Xa21 wild type; _ indicates inserted bases, and arrows indicate deletion start sites. Figure S2. Expression validation of the genes in which the differential SSRs were distributed. D62B was used as a reference sample, and the rice ubiquitin gene was used as an endogenous control. Figure S3. Development of DXB, DXT and Xa21m. O. longistaminata: Oryza longistaminata, Xa21 donor parent; IR24: Oryza sativa, recurrent parent. MAB: marker-assisted backcrossing. IRBB21, Xa21 donor parent for DXB. D62B, the recipient rice for DXB and DXT; DXB, Xa21 MAB-produced rice; DXT, Xa21 transgenic rice; Xa21m, CRISPR/Cas9-mediated Xa21 mutant rice. (PPTX 98 kb) |
