Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro
| Autor: | Marilyn Ward, E. Michelle Starke, Erin S. Gloag, Dirk de Beer, Paul Stoodley, Raja Durga Prasad Kandukuri, Sara R. Palmer, Yalda Khosravi, Arjun Chennu, Purnima S. Kumar, Sergey M. Borisov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Mechanical disruption
Oral Saliva Dental Plaque chemistry.chemical_element Dental plaque Oxygen Dissolved oxygen 03 medical and health sciences Gingivitis 0302 clinical medicine medicine Humans 030212 general & internal medicine General Dentistry Planar optodes Microspray business.industry Biofilm Microbiota Correction 030206 dentistry biochemical phenomena metabolism and nutrition medicine.disease Anoxic waters lcsh:RK1-715 chemistry Microbial population biology lcsh:Dentistry Biofilms Biophysics medicine.symptom Optode business Research Article |
| Zdroj: | BMC Oral Health BMC Oral Health, Vol 20, Iss 1, Pp 1-1 (2020) BMC Oral Health, Vol 20, Iss 1, Pp 1-14 (2020) |
| Popis: | Background Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents. Methods To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human saliva and dental plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device. Results HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qPCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions. Conclusion We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|