EHV-1 glycoprotein D (EHV-1 gD) is required for virus entry and cell-cell fusion, and an EHV-1 gD deletion mutant induces a protective immune response in mice
| Autor: | L. E. McLure, D. N. Love, J. M. Whalley, C Walker, J. E. Wellington, H. Csellner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Equine herpesvirus 1
Mutagenesis (molecular biology technique) Antibodies Viral Polymerase Chain Reaction Virus Cell Fusion Insertional mutagenesis Mice Cytopathogenic Effect Viral Viral Envelope Proteins Neutralization Tests Viral entry Virology Animals Lung Respiratory Tract Infections Cells Cultured Mice Inbred BALB C Cell fusion biology Wild type Herpesviridae Infections General Medicine biology.organism_classification Disease Models Animal Cell culture Rabbits Gene Deletion Herpesvirus 1 Equid |
| Zdroj: | Archives of Virology. 145:2371-2385 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s007050070027 |
| Popis: | Insertional mutagenesis was used to construct an equine herpesvirus 1 (EHV-1) mutant in which the open reading frame for glycoprotein D was replaced by a lacZ cassette. This gD deletion mutant (delta gD EHV-1) was unable to infect normally permissive RK cells in culture, but could be propagated in an EHV-1 gD-expressing cell line (RK/gD). Phenotypically complemented delta gD EHV-1 was able to infect RK cells, but did not spread to form syncytial plaques as seen with wild type EHV-1 or with delta gD EHV-1 infection of RK/gD cell cultures. Therefore EHV-1 gD is required for virus entry and for cell-cell fusion. The phenotypically complemented delta gD EHV-1 had very low pathogenicity in a mouse model of EHV-1 respiratory disease, compared to a fully replication-competent EHV-1 reporter virus (lacZ62/63 EHV-1). Intranasal or intramuscular inoculation of mice with delta gD EHV-1 induced protective immune responses that were similar to those elicited in mice inoculated with lacZ62/63 EHV-1 and greater than those following inoculation with UV-inactivated virus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink