Analysis of liver tissue for olestra following long-term feeding to rats and monkeys
| Autor: | F.E. Wood, B.R. DeMark, M.C. Sargent, K.C. Triebwasser, E.J. Hollenbach |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Sucrose Olestra Time Factors medicine.medical_treatment Adipose tissue Spleen Toxicology Dietary Fats Unsaturated Oral administration Internal medicine Chlorocebus aethiops medicine Toxicokinetics Animals Kidney Chemistry Fatty Acids Rats Inbred Strains General Medicine Rats Inbred F344 Rats medicine.anatomical_structure Endocrinology Liver Toxicity Injections Intravenous Female Lymph Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 29(4) |
| ISSN: | 0278-6915 |
| Popis: | The potential for olestra to be absorbed and to accumulate in tissues was investigated by analysing liver tissue from rats and monkeys in long-term feeding studies using sensitive chromatographic methods. Studies with intravenously administered olestra indicated that absorbed olestra is predominantly taken up by the liver. In monkeys, 74% of the injected dose was detected in the liver, as intact olestra, 48 hr after dosing. In rats, 58-96% of the injected dose was found in the liver, as intact olestra, within 24 hr. No olestra was detected (limit, 34 micrograms/g) in the livers of 14 monkeys fed olestra at 8% of the diet for 29 months. Also, no olestra was found in samples of liver, heart, kidney, spleen, lymph nodes and adipose tissues from 26 monkeys fed olestra at 0, 2, 4 or 6% of the diet, in random order, for consecutive 2-month periods. No olestra was detected in the livers of 47 out of 50 rats fed olestra at levels of up to 9% of the diet for up to 2 years. The amounts (2-4 micrograms/g) detected in the other three rats were near the detection limit of the chromatographic method (1.6 micrograms/g). The results show that accumulation of olestra in the liver, the primary target organ for absorbed olestra, was less than 3 x 10(-6)% of the total amount eaten by rats over 24 months and less than 4 x 10(-5)% of the amount eaten by monkeys over 29 months. These results are consistent with previous studies which showed that olestra is essentially not absorbed from the gastro-intestinal tract. |
| Databáze: | OpenAIRE |
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