Molecular cloning and sequencing of the coat protein gene of a Nebraskan isolate of tobacco necrosis virus: the deduced coat protein sequence has only moderate homology with those of strain A and strain D
| Autor: | Roy French, Lingyu Zhang, W. G. Langenberg |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Sequence alignment Genome Viral Polymerase Chain Reaction Homology (biology) Plant Viruses Open Reading Frames Tobacco necrosis virus Capsid Sequence Homology Nucleic Acid Virology Tobacco Amino Acid Sequence Cloning Molecular ORFS Peptide sequence Genetics Necrovirus Base Sequence Sequence Homology Amino Acid biology Nucleic acid sequence Genetic Variation Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Plants Toxic Open reading frame RNA Viral Capsid Proteins |
| Zdroj: | Archives of Virology. 132:291-305 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | A partial nucleotide sequence spanning the coat protein (CP) gene of a Nebraskan isolate of tobacco necrosis virus (TNV-NE) has been determined. The sequence contains at least four open reading frames (ORFs). The 5'-terminal ORF encodes a protein that has 86% and 38% homology with the polymerases of strains A (TNV-A) and D (TNV-D), respectively. The second and third ORFs probably encode 10.7 kDa and 6.2 kDa proteins (p 10.7 and p 6.2). These are respectively 90% and 96% amino acid homologous encoded by similar ORFs in TNV-A but only 26% and 20% homologous with those in TNV-D. The fourth 3'-proximal ORF encodes the 30.3 kDa CP. The amino acid sequence of TNV-NE CP is only 51% and 44% homologous to those of TNV-A and TNV-D, respectively. Thus, the CP genes of TNV-NE, TNV-A, and TNV-D are quite different. Like the sequences to the 5' side of the CP gene, that of TNV-NE is more closely related to TNV-A than to TNV-D. |
| Databáze: | OpenAIRE |
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