Molecular Investigation of Staphylococcal Cassette Chromosome Mec Types and Genotypic Relations of Methicillin-Resistant Staphylococci Isolated from Before and after Hospital Exposed Students

Autor: Gulsen Ulucam Atay, Gülçin Bayramoğlu, Tuba Gökdoğan Edgünlü, Bora Ekinci
Přispěvatelé: MÜ
Rok vydání: 2018
Předmět:
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
Microbiology (medical)
Genotype
030106 microbiology
Immunology
lcsh:QR1-502
Staphylococcus Spp
MecA
Microbial Sensitivity Tests
medicine.disease_cause
Plasmid
Microbiology
lcsh:Microbiology
SmaI
law.invention
03 medical and health sciences
methicillin resistance
Immunology and Microbiology (miscellaneous)
Staphylococcus epidermidis
law
plasmid
medicine
Pulsed-field gel electrophoresis
Humans
Immunology and Allergy
staphylococcal cassette chromosome
Polymerase chain reaction
Genetics
Pulsed-Field Gel Electrophoresis
General Immunology and Microbiology
biology
SCCmec
Staphylococcus spp
Meca
Staphylococcal Cassette Chromosome
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Infectious Diseases
pulsed-field gel electrophoresis
Staphylococcus aureus
Methicillin Resistance
Plasmids
Zdroj: Indian Journal of Medical Microbiology, Vol 36, Iss 2, Pp 257-264 (2018)
ISSN: 0255-0857
DOI: 10.4103/ijmm.ijmm_17_256
Popis: WOS: 000441827600019 PubMed ID: 30084421 Background: Reservoir of methicillin resistance genes called staphylococcal cassette chromosome mec (SCCmec), plasmids and genomic characterisations of isolates have been widely investigated in epidemiologic research. However, the extent to which these organisms are transported by patients or hospital staff is not entirely clear. Aim: This study aims to investigate the molecular relatedness and plasmid profiles of MR staphylococci isolated from nursing students before and after hospital training, to find out the possible source. Materials and Methods: This study examined 39 methicillin-resistant (MR) staphylococci and 2 inducible clindamycin-resistant, methicillin-susceptible Staphylococcus aureus. Specimens were collected before and after 4 months of hospital training from the hands and nares of 75 nursing students. A polymerase chain reaction technique was used to confirm the existence of mecA gene and identify SCCmec types; total DNA was digested by SmaI endonuclease restriction to monitorise clonal relatedness by pulsed-field gel electrophoresis (PFGE); plasmid profiles were monitorised on agarose gel. Results: All 39 isolates tested positive for mecA; SCCmec type III was observed most frequently. Interestingly, in one isolate of Staphylococcus epidermidis, four different types of SCCmec elements were observed. There were 23 different types of plasmids, whose sizes ranged from 1.4 to 46.0 kb. After PFGE dendogram analysis, two strains were classified as indistinguishable; six were closely related. Most of the isolates obtained after hospital training showed clonal similarity and seven had multiple SCCmec elements require further investigation for the possible mechanism. Conclusion: Most of the isolates obtained after hospital training showed clonal similarity and seven had multiple SCCmec elements require further investigation for the possible mechanism. Scientific Research Project Office, Mugla Sitki Kocman University, Mugla, Turkey [12/066] This work was supported by grants from Scientific Research Project Office, Mugla Sitki Kocman University, Mugla, Turkey (Project number 12/066).
Databáze: OpenAIRE
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