Multiplex Assay for Detection of Strain-Specific Antibodies against the Two Variable Regions of the G Protein of Respiratory Syncytial Virus
Autor: | Teresa C. T. Peret, Hao Qiang Zheng, Les P. Jones, Larry J. Anderson, Ruth A. Karron, Cecelia Tsou |
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Rok vydání: | 2002 |
Předmět: |
Microbiology (medical)
G protein Clinical Biochemistry Immunology Respiratory Syncytial Virus Infections Biology Antibodies Viral Vaccines Attenuated Virus Flow cytometry Mice Viral Proteins Genotype medicine Animals Humans Immunology and Allergy Multiplex Respiratory system Antigens Viral chemistry.chemical_classification Mice Inbred BALB C medicine.diagnostic_test Infant Viral Vaccines Virology Molecular biology Amino acid chemistry Child Preschool Respiratory Syncytial Virus Human biology.protein Microbial Immunology Antibody Peptides |
Zdroj: | Clinical and Vaccine Immunology. 9:633-638 |
ISSN: | 1556-679X 1556-6811 |
Popis: | The role of strain differences in respiratory syncytial virus (RSV) disease has not been clearly defined. To investigate the possibility that strain differences contribute to susceptibility to repeat infections, we developed assays to detect antibodies to the two variable regions of the RSV G protein by cloning and expressing the internal variable region at amino acids (aa) 60 to 172 (g1) and the carboxy-terminal variable region at aa 193 to the carboxy terminus (g2) from different genotypes of RSV. The purified proteins were covalently linked to beads with different proportions of red and orange fluorescent dyes and reacted against serum specimens. Antibody reacting against the differently colored beads, and thus against different G polypeptides, was detected by use of flow cytometry and the Luminex system. This assay system detected group- and, to some extent, genotype-specific responses to RSV infection and can be used to investigate the role of strain differences in RSV disease. |
Databáze: | OpenAIRE |
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