Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
| Autor: | Cody Patterson, Rayanne Pancoast, Elizabeth B Brown, Stephanie M. Rollmann |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Candidate gene lcsh:QH426-470 lcsh:Biotechnology Olfaction Biology Life history theory Eating 03 medical and health sciences RNA interference 0302 clinical medicine lcsh:TP248.13-248.65 Genetics Animals Gene Selection (genetic algorithm) 2. Zero hunger Behavior Animal Feeding Gene Expression Profiling RNA sequencing Attraction Smell lcsh:Genetics Drosophila melanogaster 030104 developmental biology Odor Odorants Drosophila DNA microarray 030217 neurology & neurosurgery Research Article Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 18, Iss 1, Pp 1-13 (2017) |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-017-4233-1 |
| Popis: | Background The olfactory system enables organisms to detect chemical cues in the environment and can signal the availability of food or the presence of a predator. Appropriate behavioral responses to these chemical cues are therefore important for organismal survival and can influence traits such as organismal life span and food consumption. However, understanding the genetic mechanisms underlying odor-guided behavior, correlated responses in other traits, and how these constrain or promote their evolution, remain an important challenge. Here, we performed artificial selection for attractive and aversive behavioral responses to four chemical compounds, two aromatics (4-ethylguaiacol and 4-methylphenol) and two esters (methyl hexanoate and ethyl acetate), for thirty generations. Results Artificial selection for odor-guided behavior revealed symmetrical responses to selection for each of the four chemical compounds. We then investigated whether selection for odor-guided behavior resulted in correlated responses in life history traits and/or food consumption. We found changes in food consumption upon selection for behavioral responses to aromatics. In many cases, lines selected for increased attraction to aromatics showed an increase in food consumption. We then performed RNA sequencing of lines selected for responses to 4-ethylguaiacol to identify candidate genes associated with odor-guided behavior and its impact on food consumption. We identified 91 genes that were differentially expressed among lines, many of which were associated with metabolic processes. RNAi-mediated knockdown of select candidate genes further supports their role in odor-guided behavior and/or food consumption. Conclusions This study identifies novel genes underlying variation in odor-guided behavior and further elucidates the genetic mechanisms underlying the interrelationship between olfaction and feeding. Electronic supplementary material The online version of this article (10.1186/s12864-017-4233-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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