Plasmid-mediated florfenicol resistance in Pasteurella trehalosi

Autor: Hayette Targant, Danièle Meunier, Stefan Schwarz, Corinna Kehrenberg, Axel Cloeckaert, Jean-Yves Madec
Přispěvatelé: Institut für Tierzucht, Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes, AFSSA, INRA, Institut National de la Recherche Agronomique (INRA), Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Microbiology (medical)
Florfenicol
Pasteurella Infections
Drug Resistance
Cattle Diseases
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Plasmid
MESH: Pasteurella
MESH: Plasmids
MESH: Anti-Bacterial Agents
Gene cluster
Drug Resistance
Bacterial
MESH: Drug Resistance
Bacterial
MESH: Cattle Diseases
Animals
Pharmacology (medical)
MESH: Animals
Pasteurella
Insertion sequence
Pasteurella multocida
030304 developmental biology
Pharmacology
Genetics
Thiamphenicol
0303 health sciences
biology
030306 microbiology
Genetic transfer
Bacterial
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Anti-Bacterial Agents
Transformation (genetics)
MESH: Cattle
MESH: Thiamphenicol
Infectious Diseases
chemistry
MESH: Pasteurella Infections
Cattle
Plasmids
Zdroj: Journal of Antimicrobial Chemotherapy
Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2006, 58 (1), pp.13-7. ⟨10.1093/jac/dkl174⟩
ISSN: 0305-7453
1460-2091
DOI: 10.1093/jac/dkl174⟩
Popis: Objectives: A florfenicol-resistant Pasteurella trehalosi isolate from a calf was investigated for the presence and the location of the gene floR. Methods: The P. trehalosi isolate 13698 was investigated for its in vitro susceptibility to antimicrobial agents and its plasmid content. A 14.9 kb plasmid, designated pCCK13698, was identified by transformation into Pasteurella multocida to mediate resistance to florfenicol, chloramphenicol and sulphonamides. The plasmid was sequenced completely and analysed for its structure and organization. Results: Plasmid pCCK13698 exhibited extended similarity to plasmid pHS-Rec from Haemophilus parasuis including the region carrying the parA, repB, rec and int genes. Moreover, it revealed similarities to plasmid RSF1010 in the parts covering the mobC and repA-repC genes and to plasmid pMVSCS1 in the parts covering the sul2-catA3-strA gene cluster. Moreover, the floR gene area corresponded to that of transposon TnfloR. In addition, two complete insertion sequences were detected that were highly similar to IS1593 from Mannheimia haemolytica and IS26 from Enterobacteriaceae. Several potential recombination sites were identified that might explain the development of plasmid pCCK13698 by recombination events. Conclusions: The results of this study showed that in the bovine pathogen P. trehalosi, floR-mediated resistance to chloramphenicol and florfenicol was associated with a plasmid, which also carried functionally active genes for resistance to sulphonamides (sul2) and chloramphenicol (catA3). This is to the best of our knowledge the first report of resistance genes in P. trehalosi and only the second report of the presence of a florfenicol-resistance gene in target bacteria of the family Pasteurellaceae.
Databáze: OpenAIRE
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