Fluorescence of blood protoporphyrin in the presence of hemoglobin
| Autor: | Robert F. Labbe, P. Sayer, M. Gouterman |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Erythrocytes
Porphyrins Erythrocyte protoporphyrin Biochemistry Hemoglobins Porphyrias chemistry.chemical_compound Methods medicine Humans Heme Whole blood Anemia Hypochromic Chromatography Spectrophotometry Atomic Iron deficiency medicine.disease Fluorescence Porphyrin Lead Poisoning Spectrometry Fluorescence chemistry Evaluation Studies as Topic Spectrophotometry Protoporphyrin Hemoglobin Mathematics |
| Zdroj: | Biochemical Medicine. 10:24-35 |
| ISSN: | 0006-2944 |
| Popis: | This study has demonstrated the feasibility of determining erythrocyte protoporphyrin concentration by the direct fluorimetric measurement of diluted whole blood. Excitation and emission wavelengths can be chosen to minimize heme (or hemoglobin) interference. Although results obtained by this method and an extraction method differed, the discrepancies were approximately constant and largely explainable on a basis of fluorescence quenching by heme. Nevertheless, several kinds of pathologic blood specimens having elevated protoporphyrin concentrations were easily detectable. Perhaps more important from a diagnostic sense, the method can be used to quantify directly the [PROTO]/[heme] ratio. This new approach to blood porphyrin assay should find extensive application in such public health problems as lead intoxication and iron deficiency. |
| Databáze: | OpenAIRE |
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