Platelet-derived growth factor receptor-α is a key determinant of smooth muscle α-actin filaments in bone marrow-derived mesenchymal stem cells
| Autor: | C. Adrian Shuttleworth, Cay M. Kielty, Stephen Ball |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male rho GTP-Binding Proteins Cell signaling Receptor Platelet-Derived Growth Factor alpha RHOA Adolescent Polymers medicine.medical_treatment Bone Marrow Cells macromolecular substances Protein Serine-Threonine Kinases Biochemistry Muscle Smooth Vascular Receptor Platelet-Derived Growth Factor beta medicine Humans Myocyte Phosphorylation Cytoskeleton Insulin-like growth factor 1 receptor rho-Associated Kinases biology Chemistry Growth factor Intracellular Signaling Peptides and Proteins Mesenchymal Stem Cells Cell Biology Coronary Vessels Actins Cell biology Actin Depolymerizing Factors biology.protein Female rhoA GTP-Binding Protein Myofibroblast Platelet-derived growth factor receptor |
| Zdroj: | The International Journal of Biochemistry & Cell Biology. 39:379-391 |
| ISSN: | 1357-2725 |
| DOI: | 10.1016/j.biocel.2006.09.005 |
| Popis: | Smooth muscle alpha-actin filaments are a defining feature of mesenchymal stem cells, and of mesenchyme-derived contractile smooth muscle cells, pericytes and myofibroblasts. Here, we show that adult bone marrow-derived mesenchymal stem cells express abundant cell surface platelet-derived growth factor receptor-alpha, having a high ratio to platelet-derived growth factor receptor-beta. Signaling through platelet-derived growth factor receptor-alpha increases smooth muscle alpha-actin filaments by activating RhoA, which results in Rho-associated kinase (ROCK)-dependent cofilin phosphorylation, enhancing smooth muscle alpha-actin filament polymerization, and also upregulates smooth muscle alpha-actin expression. In contrast, platelet-derived growth factor receptor-beta signaling strongly upregulates RhoE, which inhibits ROCK activity, promoting smooth muscle alpha-actin filament depolymerization. This study thus provides new insights into the distinct roles of platelet-derived growth factor receptor-alpha and -beta signaling in regulating the adult mesenchymal stem cell contractile cytoskeleton. |
| Databáze: | OpenAIRE |
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