Identification of a site on 18 S rRNA of human placenta ribosomes in the region of the mRNA binding center
| Autor: | D.M. Graifer, S.V. Mamaev, Alexey A. Malygin, Marina A. Zenkova, Galina G. Karpova, Mundus Da |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Uracil Nucleotides
Placenta Molecular Sequence Data Biology Ribosome 18S ribosomal RNA RNA Transfer Phe Organophosphorus Compounds Pregnancy Structural Biology RNA Ribosomal 18S Humans RNA Messenger Binding site Molecular Biology Messenger RNA Binding Sites Affinity labeling Base Sequence Affinity Labels Ribosomal RNA Molecular biology A-site Biochemistry RNA Ribosomal Nucleic Acid Conformation Female Eukaryotic Ribosome Phosphorus Radioisotopes Ribosomes |
| Zdroj: | Journal of Molecular Biology. 214:121-128 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(90)90151-b |
| Popis: | The affinity labeling of human placenta 80 S ribosomes by 4-(N-2-chloroethyl-N-methylamino) benzyl-5′-phosphamide of hexauridylate was studied. This mRNA analog has normal coding properties because its binding to placenta ribosomes significantly increases in the presence of cognate tRNAPhe. Incubation of the mRNA analog in the complex with the ribosomes and Phe-tRNAPhe leads to its covalent attachment exclusively to the small subunit (mainly to 18 S rRNA). The site of the reaction has been identified by hybridization experiments to be located within positions 975 to 1055 of 18 S rRNA. The identified fragment is located in a highly conserved part of the small subunit rRNA domain II. |
| Databáze: | OpenAIRE |
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