Comprehensive characterization of nanostructured lipid carriers using laboratory and synchrotron X-ray scattering and diffraction
| Autor: | Manfred Kriechbaum, Krunoslav Juraić, Sonja Hartl, Elisabeth Pritz, Christina Spirk, Aden Hodzic, Eva Roblegg, Carolin Tetyczka, Gerd Leitinger |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Chemistry Pharmaceutical Drug Storage Analytical chemistry Pharmaceutical Science 02 engineering and technology 030226 pharmacology & pharmacy law.invention 03 medical and health sciences Crystallinity 0302 clinical medicine Microscopy Electron Transmission X-Ray Diffraction Dynamic light scattering law Scattering Small Angle Zeta potential Lamellar structure Particle Size Drug Carriers Scattering Small-angle X-ray scattering X-ray General Medicine 021001 nanoscience & nanotechnology Lipids Domperidone Synchrotron Nanoparticles 0210 nano-technology Synchrotrons Nanostructured lipid carriers (NLC) Palmitic acid Laboratory small and wide angle X-ray scattering Synchrotron X-ray diffraction Synchrotron small angle X-ray scattering Biotechnology |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 139:153-160 |
| ISSN: | 0939-6411 |
| Popis: | The development of lipid nanoparticles requires knowledge on the crystalline structure, polymorphic transitions and lipid-drug interactions. This study aimed at introducing advanced techniques to characterize nanostructured lipid carriers (NLC) comprising palmitic acid, oleic acid, stabilizer and Domperidone. Crystallinity of single components and mixtures was investigated by laboratory Small Angle X-ray Scattering (SAXS). NLC were studied with laboratory Small and Wide Angle X-ray Scattering (SWAXS). Photon Correlation Spectroscopy and Freeze Fracture Transmission Electron Microscopy were used to monitor particle size, zeta potential and shape. Stability of NLC was investigated using synchrotron X-ray Diffraction (XRD) and SAXS and laboratory SAXS. Palmitic acid showed a lamellar structure (polymorph C), which was still present after particle preparation. Spherical 300 nm-sized particles with zeta potential values above −30 mV were obtained and Domperidone was incorporated in its amorphous form. During storage, no differences in synchrotron XRD spectra were seen. However, laboratory SAXS measurements showed a second lamellar structure, identified as polymorph B. Synchrotron SAXS temperature scans confirmed that polymorph B did not affect the morphology of the encapsulated drug or the shape of NLC. These results highlight the unique capabilities of laboratory and synchrotron X-ray Scattering and Diffraction for improved structural characterization of lipid nanoparticles. |
| Databáze: | OpenAIRE |
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