Inhibitory effects of low decibel infrasound on the cardiac fibroblasts and the involved mechanism

Autor: Qin-Qin Deng, Bao-Ying Chen, Qing Li, Zhao-Hui Pei, Zhen-Xing Lu, Haikang Zhao, Pan Chang, Jin Wei, Yu Jun
Rok vydání: 2017
Předmět:
0301 basic medicine
infrasound
030204 cardiovascular system & hematology
Rats
Sprague-Dawley
0302 clinical medicine
Transforming Growth Factor beta
Phosphorylation
Cells
Cultured
Gene knockdown
microRNA
Chemistry
Angiotensin II
lcsh:Otorhinolaryngology
lcsh:RF1-547
Blot
lcsh:Industrial medicine. Industrial hygiene
Original Article
RNA Interference
Collagen
Signal transduction
Signal Transduction
medicine.medical_specialty
Proline
Infrasound
Enzyme-Linked Immunosorbent Assay
heart
Real-Time Polymerase Chain Reaction
Tritium
Vibration
lcsh:RC963-969
03 medical and health sciences
Speech and Hearing
Internal medicine
medicine
Animals
Smad3 Protein
Cell Proliferation
Decibel
Cell growth
Myocardium
Public Health
Environmental and Occupational Health
Fibroblasts
Fibrosis
Rats
MicroRNAs
030104 developmental biology
Endocrinology
Otorhinolaryngology
Thymidine
Zdroj: Noise & Health
Noise and Health, Vol 19, Iss 88, Pp 149-153 (2017)
ISSN: 1463-1741
DOI: 10.4103/nah.nah_14_16
Popis: Introduction: Infrasound is a mechanical vibration wave with frequency between 0.0001 and 20 Hz. It has been established that infrasound of 120 dB or stronger is dangerous to humans. However, the biological effects of low decibel infrasound are largely unknown. The purpose of this study was to investigate the effects of low decibel infrasound on the cardiac fibroblasts. Materials and Methods: The cardiac fibroblasts were isolated and cultured from Sprague–Dawley rats. The cultured cells were assigned into the following four groups: control group, angiotensin II (Ang II) group, infrasound group, and Ang II+infrasound group. The cell proliferation and collagen synthesis rates were evaluated by means of [ 3 H]-thymidine and [ 3 H]-proline incorporation, respectively. The levels of TGF-β were determined by enzyme-linked immunosorbent assay. Moreover, RNAi approaches were used for the analysis of the biological functions of miR-29a, and the phosphorylation status of Smad3 was detected using western blotting analysis. Results: The results showed that low decibel infrasound significantly alleviated Ang II-induced enhancement of cell proliferation and collagen synthesis. Discussion: Compared with the control, Ang II markedly decreased the expression of miR-29a levels and increased the secretion of TGF-β and phosphorylation of Smad3, which was partly reversed by the treatment with low decibel infrasound. Importantly, knockdown of miR-29a diminished the effects of infrasound on the cardiac fibroblasts. In conclusion, low decibel infrasound inhibits Ang II-stimulated cardiac fibroblasts via miR-29a targeting TGF-β/Smad3 signaling.
Databáze: OpenAIRE
Externí odkaz: