Genotypes of hepatitis C virus in the Indian sub-continent: a decade-long experience from a tertiary care hospital in South India
Autor: | Sukanya Raghuraman, Hubert Darius J. Daniel, Jayashree Sivakumar, J Christdas, Joel David, Priya Abraham |
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Rok vydání: | 2013 |
Předmět: |
Microbiology (medical)
Genotype Hepatitis C virus lcsh:QR1-502 India Hepacivirus Biology Viral Nonstructural Proteins Chronic liver disease medicine.disease_cause Polymerase Chain Reaction lcsh:Microbiology Tertiary Care Centers chemistry.chemical_compound Hepatitis C virus genotypes medicine Humans Indian sub-continent NS5B Genotyping Retrospective Studies Molecular Epidemiology NS5B sequencing Genetic Variation Hepatitis C Sequence Analysis DNA Hepatitis C Chronic medicine.disease Virology Phylogeography chemistry treatment outcome RNA Viral Restriction fragment length polymorphism 5' Untranslated Regions Nested polymerase chain reaction Polymorphism Restriction Fragment Length |
Zdroj: | Indian Journal of Medical Microbiology, Vol 31, Iss 4, Pp 349-353 (2013) |
ISSN: | 1998-3646 |
Popis: | Background: Hepatitis C virus (HCV) is a leading cause of chronic liver disease (CLD) that can progress to cirrhosis and hepatocellular carcinoma. Genotypes of HCV can vary in pathogenicity and can impact on treatment outcome. Objectives: To study the different genotypes among patients with HCV related CLD attending a tertiary care hospital in south India during 2002-2012. Study Design: Study subjects were those referred to clinical virology from the liver clinic. Genotyping was performed using the genotype specific core primers in nested polymerase chain reaction (PCR), 5′ non-coding regions based PCR- restriction fragment length polymorphism and NS5B sequencing methods. With the latter method, obtained sequences were compared with published GenBank sequences to determine the genotype. Results: Of the 451 samples tested, HCV genotype 3 was found to be the most predominant (63.85%). Other genotypes detected were genotype 1 (25.72%), genotype 2 (0.002%), genotype 4 (7.5%) and genotype 6 (2.7%). Genotype 3 was the common genotype in patients from Eastern India while genotype 1 and 4 were mainly seen in South Indian patients. Genotype 6 was seen exclusively in patients from North-Eastern India. Two other patients were infected with recombinants of genotype 1 and 2. Conclusions: In this study spanning a decade, HCV genotype 3 and genotype 1 were found to be the predominant genotypes in the Indian sub-continent. Genotype 4 and genotype 6 appeared to show some geographic restriction. A continued monitoring of HCV genotypes is essential for the optimum management of these chronically infected patients. In addition, knowledge of circulating genotypes could impact on future vaccine formulations. |
Databáze: | OpenAIRE |
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