Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Autor: Narissara Suratannon, Massalin Nakphaichit, Wanlapa Weerapakorn, Wanwipa Vongsangnak, Suttipun Keawsompong, Nachon Raethong, Witida Sathitkowitchai
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Genes, Vol 12, Iss 331, p 331 (2021)
Genes
Volume 12
Issue 3
ISSN: 2073-4425
Popis: The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country.
Databáze: OpenAIRE
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