Cryptopatches and isolated lymphoid follicles: dynamic lymphoid tissues dispensable for the generation of intraepithelial lymphocytes
| Autor: | Sheng Yan, Reinhard Pabst, Reinhold Förster, Michaela Friedrichsen, Heiner Körner, Matthias W. Hoffmann, Tim Worbs, Oliver Pabst, Günter Bernhardt, Heike Herbrand |
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| Rok vydání: | 2004 |
| Předmět: |
Lymphotoxin-beta
Pathology medicine.medical_specialty Lymphoid Tissue Swine T cell Gut-associated lymphoid tissue Immunology Biology Lymphotoxin beta Epithelium Mice Immune system Species Specificity Intestine Small medicine Animals Humans Immunology and Allergy Lymphocytes Lymphotoxin-alpha Mice Knockout Mice Inbred BALB C Membrane Proteins Small intestine Rats Cell biology Mice Inbred C57BL Transplantation Phenotype medicine.anatomical_structure Lymphotoxin Intraepithelial lymphocyte |
| Zdroj: | European Journal of Immunology. 35:98-107 |
| ISSN: | 0014-2980 |
| DOI: | 10.1002/eji.200425432 |
| Popis: | In comparison to secondary lymphoid organs, gut-associated lymphoid tissues such as isolated lymphoid follicles (ILF) and cryptopatches (CP) have been less intensively studied. To gain a better insight into processes regulating organization and function of these structures, which are believed to participate in immune responses and extrathymic T cell development, we characterized the lymphoid structures of the murine small intestine in more detail. The size and cellular composition of small intestinal lymphoid aggregations were analyzed in C57BL/6 and BALB/c wild-type and lymphotoxin (LT)-deficient mice, by flow cytometry, histology and automated multi-color immunofluorescence microscopy evaluating large coherent areas of the intestine. These evaluations demonstrate that aggregated lymphoid structures in the small intestine vary in size and cellular composition, with a majority of structures not matching the current definitions of CP or ILF. Accordingly, significant variations depending on species, age and mouse strain were observed. Furthermore, small bowel transplantation revealed a rapid exchange of B but not T cells between host and grafted tissue. Moreover, LT-deficient animals lack any intestinal lymphoid aggregations yet possess the complete panel of intraepithelial lymphocytes (IEL). In summary, our observations disclose intestinal lymphoid aggregations as dynamic structures with a great deal of inborn plasticity and demonstrate their dispensability for the generation of IEL. |
| Databáze: | OpenAIRE |
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