Single-image axial localization precision analysis for individual fluorophores
| Autor: | Michael C. DeSantis, Robert E. Blankenship, Yan Mei Wang, Shannon Kian Zareh, Xianglu Li |
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| Rok vydání: | 2012 |
| Předmět: |
Fluorophore
Gaussian Biophysics 01 natural sciences Upper and lower bounds Standard deviation 010309 optics 03 medical and health sciences symbols.namesake chemistry.chemical_compound 0302 clinical medicine Optics Imaging Three-Dimensional ocis:(100.6890) Three-dimensional image processing 0103 physical sciences Image Interpretation Computer-Assisted ocis:(180.6900) Three-dimensional microscopy 030304 developmental biology Fluorescent Dyes Physics 0303 health sciences Total internal reflection Millisecond business.industry Expression (mathematics) Atomic and Molecular Physics and Optics Photon counting Intensity (physics) ocis:(100.6640) Superresolution Spectrometry Fluorescence chemistry Microscopy Fluorescence Temporal resolution ocis:(180.2520) Fluorescence microscopy symbols Research-Article ocis:(110.2960) Image analysis business 030217 neurology & neurosurgery Algorithms |
| Zdroj: | Optics Express |
| ISSN: | 1094-4087 |
| Popis: | Bio-mechanism investigations demand single particle tracking with high spatial and temporal resolutions which require single fluorophore 3D localization measurements with matching precision and speed. Although the precision for lateral-localization measurements is well described by an analytical expression, for the axial direction, it is often obtained by repeating location measurements or by estimating a lower bound. Here, we report a precision expression for an axial-localization method that analyzes the standard deviations of single fluorophores' intensity profiles. Like the lateral-localization precision, this expression includes all relevant experimental effects measurable from a Gaussian intensity profile of the fluorophore. This expression completes the precision analysis for single-image 3D localization of individual fluorophores and lifts the temporal resolution to the typical exposure timescales of milliseconds. |
| Databáze: | OpenAIRE |
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