Transcriptional signatures of Zika virus infection in astrocytes
| Autor: | Dawn M. Szeltner, Nicholas J. Maness, Andrew G. MacLean, Melody Baddoo, Tiffany A. Peterson, Elizabeth A. Scheef, Blake Schouest, Erik K. Flemington, Nathan Ungerleider |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lineage (genetic) Virus Article Zika virus 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Virology Lipid biosynthesis medicine Animals Nonhuman primates Cells Cultured Infectivity biology Zika Virus Infection Brain RNA sequencing Zika Virus biology.organism_classification Macaca mulatta Nonhuman primate 030104 developmental biology medicine.anatomical_structure Neurology Astrocytes Neurology (clinical) Transcriptome 030217 neurology & neurosurgery Hypoxic stress Astrocyte |
| Zdroj: | Journal of Neurovirology |
| ISSN: | 1538-2443 1355-0284 |
| Popis: | Astrocytes are an early and important target of Zika virus (ZIKV) infection in the developing brain, but the impacts of infection on astrocyte function remain controversial. Given that nonhuman primate (NHP) models of ZIKV infection replicate aspects of neurologic disease seen in human infections, we cultured primary astrocytes from the brain tissue of infant rhesus macaques and then infected the cells with Asian or African lineage ZIKV to identify transcriptional patterns associated with infection in these cells. The African lineage virus appeared to have greater infectivity and promote stronger antiviral signaling, but infection by either strain ultimately produced typical virus response patterns. Both viruses induced hypoxic stress, but the Asian lineage strain additionally had an effect on metabolic and lipid biosynthesis pathways. Together, these findings describe an NHP astrocyte model that may be used to assess transcriptional signatures following ZIKV infection. |
| Databáze: | OpenAIRE |
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