Genome sequence analysis of 91 Salmonella Enteritidis isolates from mice caught on poultry farms in the mid 1990s
| Autor: | Guojie Cao, Yan Luo, Marc W. Allard, Jean Guard, Maria Hoffmann, Sherrill Davison, Rebecca L. Bell, Jie Zheng, Kuan Yao, Eric W. Brown, Guodong Zhang, Joseph D. Baugher, Nathan Likens |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Salmonella Farms Salmonella enteritidis Minisatellite Repeats Biology medicine.disease_cause Polymorphism Single Nucleotide 01 natural sciences Genome Poultry Mice 03 medical and health sciences Genotype-phenotype distinction INDEL Mutation Genotype Genetics medicine Animals ORFS Phylogeny 030304 developmental biology Whole genome sequencing 0303 health sciences Mutation Whole Genome Sequencing Phenotype Algorithms Genome Bacterial 010606 plant biology & botany |
| Zdroj: | Genomics. 112:528-544 |
| ISSN: | 0888-7543 |
| Popis: | A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995–1998. Isolates separated into two genomic clades and 12 subgroups due to 742 mutations. Nineteen ORF-disruptive mutations were found, and in addition, bigA had exceptional heterogeneity requiring additional evaluation. The TRAMS algorithm detected only 6 ORF disruptions. The sefD mutation was the most frequently encountered mutation and it was prevalent in human, poultry, environmental and mouse isolates. These results confirm previous assessments of the mouse as a rich source of Salmonella enterica serovar Enteritidis that varies in genotype and phenotype. |
| Databáze: | OpenAIRE |
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