KITENIN promotes glioma invasiveness and progression, associated with the induction of EMT and stemness markers
Autor: | Hyang-Hwa Ryu, Kyung-Keun Kim, Young-Eun Joo, Ok Kim, Kyung-Hwa Lee, Jae Hyuk Lee, Se-Jeong Oh, Eun-Jung Ahn, Shin Jung, Kyung-Sub Moon, Somy Yoon, Jeong-A Bae |
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Rok vydání: | 2014 |
Předmět: |
Male
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Time Factors Neoplastic stem cell Neoplasm invasiveness Mice Nude Biology Transfection Metastasis Cell Movement Glioma Cell Line Tumor medicine Biomarkers Tumor Animals Humans University medical In patient Mouse tumor Epithelial–mesenchymal transition Mice Inbred BALB C Human VANGL1 protein Brain Neoplasms Membrane Proteins Middle Aged medicine.disease Up-Regulation Mice Inbred C57BL Oncology Neoplasm Invasiveness embryonic structures Cancer research Neoplastic Stem Cells Female RNA Interference Stem cell Carrier Proteins Signal Transduction Research Paper |
Zdroj: | Oncotarget |
ISSN: | 1949-2553 |
Popis: | // Kyung-Hwa Lee 1,* , Eun-Jung Ahn 2,* , Se-Jeong Oh 1 , Ok Kim 1 , Young-Eun Joo 3 , Jeong-A Bae 4 , Somy Yoon 4 , Hyang-Hwa Ryu 2 , Shin Jung 2 , Kyung-Keun Kim 4 , Jae-Hyuk Lee 1 and Kyung-Sub Moon 2 1 Department of Pathology, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Jeollanam-do, South Korea 2 Department of Neurosurgery, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Jeollanam-do, South Korea 3 Department of Internal Medicine, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Jeollanam-do, South Korea 4 Medical Research Center of Gene Regulation and Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju, South Korea * These authors contributed equally to this work Correspondence: Kyung-Sub Moon, email: // Keywords : Neoplastic stem cell, Epithelial-mesenchymal transition, Glioma, Neoplasm invasiveness, Human VANGL1 protein Received : October 21, 2014 Accepted : December 18, 2014 Published : December 26, 2014 Abstract KITENIN (KAI1 COOH-terminal interacting tetraspanin) promotes tumor invasion and metastasis in various cancers. This study assessed the association between KITENIN expression and advanced glioma grade in patients. In vitro assays revealed that KITENIN knockdown inhibited the invasion and migration of glioma cells, whereas KITENIN overexpression promoted their invasion and migration. In orthotopic mouse tumor models, mice transplanted with KITENIN-transfected glioma cells had significantly shorter survival than mice transplanted with mock-transfected cells. Patients with low KITENIN expression showed a significantly longer progression-free survival than patients with high KITENIN expression. KITENIN induced the expression of the epithelial-mesenchymal transition (EMT) markers (N-cadherin, ZEB1, ZEB2, SNAIL and SLUG) as well as the glioma stemness markers (CD133, ALDH1 and EPH-B1). Taken together, these findings showed that high levels of KITENIN increased glioma invasiveness and progression, associated with the up-regulation of EMT and stemness markers. |
Databáze: | OpenAIRE |
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