Characteristic differences in radiation‐induced DNA damage response in human papillomavirus‐negative and human papillomavirus‐positive head and neck cancers with accumulation of fractional radiation dose
| Autor: | Alexander H. Staudacher, Eva Bezak, Paul Reid, Ian N. Olver, Leyla Moghaddasi, Michael P. Brown, Loredana G. Marcu |
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| Přispěvatelé: | Reid, Paul, Staudacher, Alexander H, Marcu, Loredana G, Olver, Ian, Moghaddasi, Leyla, Brown, Michael P, Bezak, Eva |
| Rok vydání: | 2021 |
| Předmět: |
Human Papillomavirus Positive
HPV DNA repair medicine.medical_treatment Alphapapillomavirus Radiation Dosage HNSCC Cell Line Tumor Humans Medicine γH2AX Papillomaviridae Cumulative dose business.industry Papillomavirus Infections Human Papillomavirus Negative medicine.disease Head and neck squamous-cell carcinoma Radiation therapy stomatognathic diseases Otorhinolaryngology Head and Neck Neoplasms fractionated radiation therapy micronuclei Cancer research Biomarker (medicine) Radiation Induced DNA Damage business DNA Damage |
| Zdroj: | Head & Neck. 43:3086-3096 |
| ISSN: | 1097-0347 1043-3074 |
| DOI: | 10.1002/hed.26802 |
| Popis: | Background: Superior treatment responses by patients with human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC),compared to patients with HNSCC from other causes, drive biomarker research to optimize treatment. Most HNSCC patients receive radiation therapy delivered as a fractionated course. Changing HPV status in HNSCC from apositive prognostic marker to a predictive one requires biomarkers that capture cellular radiation response to cumulative dose.Methods: Nuclear enlargement, γH2AX expression and micronuclei count,were studied in six HNSCC cell lines after 4 Gy fractionated X-irradiation. Results: All HNSCC cell lines displayed altered cellular responses, indicating increasing inability to repair radiation damage with subsequent radiation fractions.Increases in nuclear area were significantly greater among HPV positive cell lines(207% and 67% for the HPV positive and HPV negative groups, respectively). Conclusions: A different character of DNA repair dysfunction in the HPV positive group suggests greater chromosomal translocation with accumulated radiation dose. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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