IVS1 -397TC estrogen receptor α polymorphism is associated with low-grade systemic inflammatory response in type 1 diabetic girls
| Autor: | Monika Ryba-Stanisławowska, Agnieszka Brandt, Jolanta Myśliwska, Małgorzata Myśliwiec, Karolina Rybarczyk-Kapturska |
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| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Vascular Endothelial Growth Factor A medicine.medical_specialty Article Subject Adolescent Genotype medicine.medical_treatment Immunology Estrogen receptor Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Nephropathy Polymorphism (computer science) Internal medicine lcsh:Pathology medicine Humans Interleukin 6 Inflammation Type 1 diabetes Estradiol Interleukin-6 Tumor Necrosis Factor-alpha Microcirculation Estrogen Receptor alpha Forkhead Transcription Factors Cell Biology medicine.disease Interleukin-10 Interleukin 10 Cytokine Endocrinology Diabetes Mellitus Type 1 Case-Control Studies biology.protein Cytokines Th17 Cells Female Estrogen receptor alpha lcsh:RB1-214 Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2014 (2014) |
| ISSN: | 1466-1861 |
| Popis: | Purpose. The study aimed to investigate the influence of estrogen receptorα(ER-α) genotypes on inflammatory response and development of microvascular complications in girls with type 1 diabetes.Methods. 152 young regularly menstruating girls with diagnosed type 1 diabetes and 84 young, healthy menstruating girls were recruited. ER-αgenotyping was carried out by PCR. Serum concentrations of 17β-estradiol, as well as IL-6, TNF-α, VEGF, and IL-10, were measured. CD4+Foxp3+TH17 cells were isolated and analyzed by flow cytometry.Results. Type 1 diabetic girls carrying TT genotype were characterized by the lowest serum estradiol level and IL-10 and highest IL-6, TNF-α, and VEGF. The association between the level of certain cytokine and the genetic variant of estrogen receptorαpolymorphism was analyzed. Frequencies of CD4+Foxp3+TH17 cells were also enhanced in TT bearing girls with type 1 diabetes and correlated with the level of analyzed cytokines. In addition, the correlation between serum estradiol level and cytokine concentrations was observed.Conclusions. We propose that TT variant of estrogen receptorαpolymorphism may be associated with enhanced inflammatory response, which in turn may lead to acceleration of diabetic retino- and nephropathy in girls with type 1 diabetes. This finding may help the physicians to predict the onset and progression of diabetic microvascular complications. |
| Databáze: | OpenAIRE |
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