Osteoblastic cell behavior and gene expression related to bone metabolism on different titanium surfaces
| Autor: | Eugenio Velasco-Ortega, Isabel Fos-Parra, Daniel Cabanillas-Balsera, Javier Gil, Iván Ortiz-García, Mercè Giner, Jesús Bocio-Núñez, María-José Montoya-García, Álvaro Jiménez-Guerra |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Estomatología, Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica, Universidad de Sevilla. Departamento de Medicina, Ministerio de Ciencia e Innovación. España |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Cell viability
Roughness and wettability 616.3 Rugosidad y humectabilidad surfaces Viabilidad celular Catalysis Superfícies Osteoblastos Inorganic Chemistry titanium Physical and Theoretical Chemistry Molecular Biology Spectroscopy cell viability Titanium Osteoblasts Organic Chemistry roughness and wettability Viabilitat cel·lular osteoblasts General Medicine Titani Expressió gènica Computer Science Applications Surfaces Titanio Rugositat i humectabilitat Superficies gene expression Gene expression Expresión génica |
| Zdroj: | International Journal of Molecular Sciences Volume 24 Issue 4 Pages: 3523 |
| Popis: | The surface topography of titanium dental implants has a great influence on osseointegration. In this work, we try to determine the osteoblastic behavior and gene expression of cells with different titanium surfaces and relate them to the physicochemical properties of the surface. For this purpose, we have used commercial titanium discs of grade 3: as-received corresponds to machined titanium without any surface treatment (MA), chemically acid etched (AE), treated via sand blasting with Al2O3 particles (SB) and a sand-blasting treatment with acid etching (SB+AE). The surfaces have been observed using scanning electron microscopy (SEM) and the roughness, wettability and surface energy with dispersive and polar components have been characterized. Osteoblastic cultures were performed with SaOS-2 osteoblastic cells determining cell viability as well as alkaline phosphatase levels for 3 and 21 days, and osteoblastic gene expression was determined. The roughness values of the MA discs was 0.02 μm, which increases to 0.3 μm with acid attack and becomes the maximum for the sand-blasted samples, reaching values of 1.2 μm for SB and SB+AE. The hydrophilic behavior of the MA and AE samples with contact angles of 63° and 65° is superior to that of the rougher samples, being 75° for SB and 82° for SB+AE. In all cases, they show good hydrophilicity. GB and GB+AE surfaces present a higher polar component in the surface energy values, 11.96 and 13.18 mJ/m2, respectively, than AE and MA, 6.64 and 9.79 mJ/m2, respectively. The osteoblastic cell viability values at three days do not show statistically significant differences between the four surfaces. However, the viability of the SB and SB+AE surfaces at 21 days is much higher than that of the AE and MA samples. From the alkaline phosphatase studies, higher values were observed for those treated with sand blasting with and without acid etching compared to the other two surfaces, indicating a greater activity in osteoblastic differentiation. In all cases except in the Osterix (Ostx) —osteoblast-specific transcription factor—a decrease in gene expression is observed in relation to the MA samples (control). The most important increase was observed for the SB+AE condition. A decrease in the gene expression of Osteoprotegerine (OPG), Runt-related transcription factor 2 (Runx2), Receptor Activator of NF-κB Ligand (RANKL) and Alkaline Phosphatase (Alp) genes was observed in the AE surface. |
| Databáze: | OpenAIRE |
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