Effects of Mycophenolate Mofetil on Chronic Allograft Nephropathy by Affecting RHO/ROCK Signal Pathways

Autor: Xuelei Ma, Lu Yang, Yujun Shi, J. Song, Q.J. Xia, Yiping Lu, Y. Li, G.H. Luo
Rok vydání: 2008
Předmět:
Zdroj: Transplantation Proceedings. 40:2790-2794
ISSN: 0041-1345
DOI: 10.1016/j.transproceed.2008.08.003
Popis: Aim. We sought to investigate the effects of mycophenolate mofetil (MMF) on chronic allograft nephropathy (CAN) by affecting Rho and ROCK signal pathways. Methods. Male inbred F344 rat renal grafts orthotopically transplanted into Lewis rats were first treated with CsA (10 mg/kg -1 · d -1 X 10 d) and then divided into 3 groups (each n = 9): (1) orally vehicle, (2) cyclosporine (CsA, 6 mg/kg -1 · d -1 ) and (3) MMF (20 mg/kg -1 · d -1 ). In addition we performed autografts of F344 (n = 10) at 4, 8, and 12 weeks, serum creatinine (SCr) was measured and pathologic changes assessed. Expression of RhoA and ROCK-1 was determined by real-time reverse transcriptase polymerase chain reaction. Expressions of α-smooth muscle actin (a-SMA) and connective tissue growth factor (CTGF) were observed by immunohistochemistry. Results. SCr and Banff score began to increase at 4 weeks in all 3 allografted groups with obvious deterioration in both the vehicle and CsA groups at 8 and 12 weeks. The differences between vehicle/CsA and autografts were significant (P =.000). SCr and Banff score among the MMF group increased mildly and moderately at 8 and 12 weeks, respectively, but were significantly lower than those in the vehicle/CsA cohort (P
Databáze: OpenAIRE
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