Comparative studies on cell lines established from normal and radiation-exposed miniature swine
| Autor: | M. J. Hooper, M.E. Frazier, T.K. Andrews, R.N. Ushijima |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Myeloid
Swine Aneuploidy Miniature swine Plant Science Biology Cell Line Cornea Mice Myeloproliferative Disorders Metaplasia medicine Animals Leukemia Radiation-Induced Contact inhibition Neoplasms Experimental medicine.disease Molecular biology Clone Cells Radiation Injuries Experimental Leukemia Cell Transformation Neoplastic medicine.anatomical_structure Leukemia Myeloid Primary Myelofibrosis Cell culture Karyotyping Immunology Strontium Radioisotopes medicine.symptom Cell Division Biotechnology |
| Zdroj: | In Vitro. 15:1001-1012 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02619159 |
| Popis: | Cloned cell lines were established from two swine with radiation-induced myeloproliferative disorders, including one cell culture from an animal with myelogenous leukemia and one from an animal with myeloid metaplasia. A third cloned cell line with similar morphology was established from pooled normal fetal swine cornea to compare the growth characteristics of cells from normal and irradiated swine. All three cell lines grew as foci of aggregated cells and were able to form macroscopic colonies in semisolid agar medium. The lack of normal mechanisms of contact inhibition and the observed aneuploidy indicated that these cells were morphologically transformed. Further, the cloned cells caused tumors in nude mice, clearly indicating that these cells were also malignantly transformed. A major difference between these cell lines was that type C viruses were observed only in the cells derived from swine with myeloproliferative disorders. |
| Databáze: | OpenAIRE |
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