The extracellular polysaccharide of Pichia (Hansenula) holstii NRRL Y-2448: The structure of the phosphomannan backbone
| Autor: | Klaus Bock, Jens Ø. Duus, Lesley A.S. Parolis, Morten Meldal, Haralambos Parolis |
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| Rok vydání: | 1996 |
| Předmět: |
Models
Molecular Magnetic Resonance Spectroscopy Stereochemistry Molecular Sequence Data Molecular Conformation Oligosaccharides Sequence (biology) Polysaccharide Biochemistry Pichia Phosphates Analytical Chemistry Mannans chemistry.chemical_compound Hydrolysis Repetitive Sequences Nucleic Acid chemistry.chemical_classification Chromatography Molecular Structure biology Polysaccharides Bacterial Organic Chemistry General Medicine Nuclear magnetic resonance spectroscopy Oligosaccharide biology.organism_classification Carbohydrate Sequence chemistry Extracellular Space Two-dimensional nuclear magnetic resonance spectroscopy Derivative (chemistry) |
| Zdroj: | Carbohydrate Research. 293:101-117 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/0008-6215(96)00190-5 |
| Popis: | The phosphomannan core of the exopolysaccharide of Pichia (Hansenula) holstii NRRL Y-2448 was isolated after hydrolytic removal of the oligosaccharide phosphate side-chains. The core polysaccharide and its dephosphorylated derivative were subjected to extensive 1D and 2D NMR spectroscopy which yielded information on the linkage sites and on the sequence of the mannosyl residues in the major oligosaccharide repeating unit. The most probable structure for the repeating unit was -[6-O-PO 3 H 2 -α- D -Man-(1→3)-α- D -Man-(1→2)-α- D -Man-(1→2)]-α- D -Man-(1→6)-[-α- D -Man-(1→2)]-α- D -Man-(1→6)- A semiquantitative conformational analysis was performed by Monte Carlo simulations and the result was confirmed by comparison with the experimentally determined NMR data. The distance distribution for the phosphate groups was determined from the modelling and was found to cover the expected range of distances for phosphorylated high-mannose oligosaccharides. |
| Databáze: | OpenAIRE |
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