Characterization of hematopoietic stem cell subsets from patients with multiple myeloma after mobilization with plerixafor
| Autor: | Isabelle Hellwig, Patrick Wuchter, Thomas Bruckner, Anthony D. Ho, Isabel Taubert, Rainer Saffrich, Abraham Zepeda-Moreno, Volker Eckstein |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Benzylamines Receptors CXCR4 Cancer Research Immunology CD34 Biology Cyclams Polymerase Chain Reaction Autologous stem-cell transplantation Heterocyclic Compounds medicine Humans Immunology and Allergy Cells Cultured Genetics (clinical) Multiple myeloma Aged Transplantation Plerixafor Hematopoietic stem cell Cell Biology Middle Aged Hematopoietic Stem Cells medicine.disease Hematopoietic Stem Cell Mobilization Granulocyte colony-stimulating factor Haematopoiesis medicine.anatomical_structure Oncology Cancer research Female Stem cell Multiple Myeloma medicine.drug |
| Zdroj: | Cytotherapy. 13:459-466 |
| ISSN: | 1465-3249 |
| Popis: | Background aims. Previous studies have demonstrated that the combination of granulocyte – colony-stimulating factor (G-CSF) plerixafor is more effi cient in mobilizing CD34 hematopoietic stem cells (HSC) into the peripheral blood than G-CSF alone. In this study we analyzed the impact of adding plerixafor to G-CSF upon the mobilization of different HSC subsets. Methods. We characterized the immunophenotype of HSC subsets isolated from the peripheral blood of eight patients with multiple myeloma (MM) before and after treatment with plerixafor. All patients were supposed to collect stem cells prior to high-dose chemotherapy and consecutive autologous stem cell transplantation, and therefore received front-line mobilization with 4 days of G-CSF followed by a single dose of plerixafor. Samples of peripheral blood were analyzed comparatively by flcytometry directly before and 12 h after administration of plerixafor. Results. The number of aldehyde dehydrogenase (ALDH) bright and CD34 cells was signifi cantly higher after plerixafor treatment (1.2 – 5.0 and 1.5 – 6.0 times; both P 0.01) and an enrichment of the very primitive CD34 CD38 – and ALDH bright CD34 CD38 – HSC subsets was detectable. Additionally, two distinct ALDH subsets could be clearly distinguished. The small ALDH high subset showed a higher number of CD34 CD38 – cells in contrast to the total ALDH bright subpopulation and probably represented a very primitive subpopulation of HSC. Conclusions. A combined staining of ALDH, CD34 and CD38 might represent a powerful tool for the identifi cation of a very rare and primitive hematopoietic stem cell subset. The addition of plerixafor mobilized not only more CD34 cells but was also able to increase the proportion of more primitive stem cell subsets. |
| Databáze: | OpenAIRE |
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