High Resolution Human Leukocyte Antigen Class I Allele Frequencies and HIV-1 Infection Associations in Chinese Han and Uyghur Cohorts
| Autor: | Yongtao Sun, Yanhou Liu, Qi Liao, Yichen Lu, Nicholas Kushner, Zhongfang Zhao, Yiming Shao, Neal Touzjian, Tianyi Li, Amie J. Strong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Viral Diseases China Adolescent Genotype Immunology Sexually Transmitted Diseases lcsh:Medicine HIV Infections Human leukocyte antigen Biology Microbiology Major Histocompatibility Complex Immunodeficiency Viruses Asian People Gene Frequency Polymorphism (computer science) Virology Humans Genetic Predisposition to Disease Typing Allele lcsh:Science Allele frequency Alleles Aged Genetics Multidisciplinary Polymorphism Genetic Histocompatibility Testing lcsh:R Haplotype Histocompatibility Antigens Class I HIV Middle Aged Chinese people AIDS Infectious Diseases Haplotypes Cohort HIV-1 Medicine lcsh:Q Clinical Immunology Female Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 12, p e50656 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Background Host immunogenetic factors such as HLA class I polymorphism are important to HIV-1 infection risk and AIDS progression. Previous studies using high-resolution HLA class I profile data of Chinese populations appeared insufficient to provide information for HIV-1 vaccine development and clinical trial design. Here we reported HLA class I association with HIV-1 susceptibility in a Chinese Han and a Chinese Uyghur cohort. Methodology/Principal Findings Our cohort included 327 Han and 161 Uyghur ethnic individuals. Each cohort included HIV-1 seropositive and HIV-1 seronegative subjects. Four-digit HLA class I typing was performed by sequencing-based typing and high-resolution PCR-sequence specific primer. We compared the HLA class I allele and inferred haplotype frequencies between HIV-1 seropositive and seronegative groups. A neighbor-joining tree between our cohorts and other populations was constructed based on allele frequencies of HLA-A and HLA-B loci. We identified 58 HLA-A, 75 HLA-B, and 32 HLA-Cw distinct alleles from our cohort and no novel alleles. The frequency of HLA-B*5201 and A*0301 was significantly higher in the Han HIV-1 negative group. The frequency of HLA-B*5101 was significantly higher in the Uyghur HIV-1 negative group. We observed statistically significant increases in expectation-maximization (EM) algorithm predicted haplotype frequencies of HLA-A*0201-B*5101 in the Uyghur HIV-1 negative group, and of Cw*0304-B*4001 in the Han HIV-1 negative group. The B62s supertype frequency was found to be significantly higher in the Han HIV-1 negative group than in the Han HIV-1 positive group. Conclusions At the four-digit level, several HLA class I alleles and haplotypes were associated with lower HIV-1 susceptibility. Homogeneity of HLA class I and Bw4/Bw6 heterozygosity were not associated with HIV-1 susceptibility in our cohort. These observations contribute to the Chinese HLA database and could prove useful in the development of HIV-1 vaccine candidates. |
| Databáze: | OpenAIRE |
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