Gene-to-Gene Network Analysis of the Mediation of Plant Innate Immunity by the Eliciting Plant Response-Like 1 (Epl1) Elicitor of Trichoderma formosa
| Autor: | Chi Hua Cheng, Hsin Yi Wu, Shih-Shun Lin, Bing Nan Shen, Yu Tai Wang, Li-Yu Daisy Liu, Hao Ching Wang, Yan Huey Chen, Sin Fen Hu, Fang Fang Chen, Chaur Tsuen Lo, Qian Wen Shang, Kou-Cheng Peng |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Models Molecular Physiology Protein Conformation Plant Immunity Nicotiana benthamiana Biology 01 natural sciences Deep sequencing Transcriptome Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Gene Expression Regulation Fungal Tobacco Tomato mosaic virus Gene Regulatory Networks DNA Fungal Phylogeny Plant Diseases Plant Proteins Genetics Trichoderma Innate immune system Base Sequence Jasmonic acid fungi food and beverages General Medicine biology.organism_classification Immunity Innate Elicitor 030104 developmental biology chemistry Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 31(7) |
| ISSN: | 0894-0282 |
| Popis: | A new clade, Trichoderma formosa, secretes eliciting plant response-like 1 (Epl1), a small peptide elicitor that stimulates plant immunity. Nicotiana benthamiana pretreated with Epl1 for 3 days developed immunity against Tomato mosaic virus (ToMV) infection. The transcriptome profiles of T. formosa and N. benthamiana were obtained by deep sequencing; the transcript of Epl1 is 736 nt in length and encodes a 12-kDa peptide. Identifying critical genes in Epl1-mediated immunity was challenging due to high similarity between the transcriptome expression profiles of Epl1-treated and ToMV-infected N. benthamiana samples. Therefore, an efficient bioinformatics data mining approach was used for high-throughput transcriptomic assays in this study. We integrated gene-to-gene network analysis into the ContigViews transcriptome database, and genes related to jasmonic acid and ethylene signaling, salicylic acid signaling, leucine-rich repeats, transcription factors, and histone variants were hubs in the gene-to-gene networks. In this study, the Epl1 of T. formosa triggers plant immunity against various pathogen infections. Moreover, we demonstrated that high-throughput data mining and gene-to-gene network analysis can be used to identify critical candidate genes for further studies on the mechanisms of plant immunity. |
| Databáze: | OpenAIRE |
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