Spontaneous secretion of interleukin-17 and -22 by human cervical cells in Chlamydia trachomatis infection
| Autor: | Rajneesh Jha, Aruna Mittal, Axel Finckh, Cem Gabay, Sylvette Bas, Sudha Salhan, Pragya Srivastava |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Receptors CCR6 Adult medicine.medical_treatment Interferon-gamma/analysis Immunology Chlamydia trachomatis Cervix Uteri Biology medicine.disease_cause Microbiology Peripheral blood mononuclear cell Interleukin 22 Interferon-gamma Young Adult Immune system medicine Humans Interleukins/analysis/secretion Chlamydiaceae Cervix Cells Cultured ddc:616 Interleukins Interleukin-17 Chlamydia Infections biology.organism_classification Flow Cytometry Cervix Uteri/immunology/secretion Chlamydia Infections/immunology Chlamydia trachomatis/physiology Infectious Diseases medicine.anatomical_structure Cytokine Cross-Sectional Studies Gene Expression Regulation Interleukin-17/analysis/secretion CD4-Positive T-Lymphocytes/immunology Female Receptors CCR6/immunology Interleukin 17 |
| Zdroj: | Microbes and Infection, Vol. 13, No 2 (2011) pp. 167-78 |
| ISSN: | 1286-4579 |
| Popis: | To investigate whether IL-17A (IL-17) and IL-22 are produced in response to Chlamydia trachomatis infection, the cervical washes of 27 women with C. trachomatis infection and 17 C. trachomatis negative controls were collected. The levels of cytokines were determined in the cervical wash and in the supernatant of cervical and systemic cell cultures upon C. trachomatis antigen stimulation. C. trachomatis infection appeared to activate local IL-17 and IL-22 production more efficiently than IFN-γ production. In the cervical wash of infected women, median concentrations of IL-17 and -22 were 5- and 3-fold higher, respectively, than in negative controls. The spontaneous intracellular expression of these cytokines was analysed by flow cytometry in blood and cervical cells and 26% of cervical mononuclear cells from infected women were shown to produce IL-22 and 12% to coproduce IL-17 and IL-22. In addition, it was demonstrated that 20-25% of IL-22 producing and IL-17-IL-22 coproducing cervical CD4+ T cells expressed the mucosal homing receptor CCR6. These results suggest that CCR6 is involved in the migration of these cells to the cervix and that IL-17 and IL-22 might play a role in the immune response at the site of C. trachomatis infection. |
| Databáze: | OpenAIRE |
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