The expression of cytokine and its significance for the intervertebral disks of Kazakhs

Autor: Miao Xiaogang, Zhang Liping, Habaxi Kaken, Hou Quanshan
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Microbiology (medical)
Adult
Male
Pathology
medicine.medical_specialty
China
medicine.medical_treatment
Clinical Biochemistry
Intervertebral Disc Degeneration
Spinal disease
Proinflammatory cytokine
Cohort Studies
03 medical and health sciences
Young Adult
0302 clinical medicine
Lumbar
medicine
cytokine
Ethnicity
Immunology and Allergy
Humans
Statistical analysis
030212 general & internal medicine
Patient group
Intervertebral Disc
Research Articles
Aged
business.industry
Interleukin-6
Biochemistry (medical)
Public Health
Environmental and Occupational Health
Hematology
intervertebral disk herniation
Middle Aged
medicine.disease
Interleukin-10
Medical Laboratory Technology
Intervertebral disk
Cytokine
Immunohistochemistry
Female
business
030217 neurology & neurosurgery
Intervertebral Disc Displacement
Research Article
Diskectomy
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
Popis: Background Proinflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10 are expressed in herniated intervertebral disks of Kazakhs, but their significance is not yet understood. Methods Specimens of herniated lumbar disk were collected from 30 patients with single disk herniation during lumbar discectomy. As a control, 10 specimens were collected postmortem from the cadavers of individuals who did not die from spinal disease. The clinical symptoms in all cases were evaluated preoperation by the JOA index. The expressions of IL-6 and IL-10 in the degenerative disk cells of each specimen were detected using immunohistochemical staining. The amounts of IL-6 and IL-10 in each intervertebral disk were assessed by enzyme-linked immunosorbent assay. Results The expression levels of IL-6 and IL-10 were significantly higher in the patient group than in the control group. Statistical analysis showed differences between ethnic Kazakh and Han patients in both the expression of IL-6 and JOA scores. Immunohistochemical staining showed that in the patient group, IL-6 was found in 28 patients and IL-10 was found in 27 patients. Conclusion The high expression of cytokines, such as IL-6 and IL-10, has an important relationship with disk degeneration. Of course, age is another factor in cell apoptosis of degenerative disks. More research is necessary to discover how ethnicity and heredity impact the levels of expressed cytokines.
Databáze: OpenAIRE
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