Protein engineering of a human enzyme that hydrolyzes V and G nerve agents: design, construction and characterization
| Autor: | Charles B. Millard, Clarence A. Broomfield, Oksana Lockridge |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Butyrylthiocholine
Echothiophate Iodide Soman Mutant Protein Engineering Torpedo Toxicology Serine Benzoylcholine Animals Humans Chemical Warfare Agents Binding site Cephamycins Site-directed mutagenesis Butyrylcholinesterase chemistry.chemical_classification Binding Sites biology Hydrolysis Active site Organothiophosphorus Compounds General Medicine Protein engineering Sarin Organophosphates Kinetics Enzyme chemistry Biochemistry Drug Design Inactivation Metabolic Mutagenesis Site-Directed biology.protein Cholinesterase Inhibitors |
| Zdroj: | Chemico-Biological Interactions. :413-418 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/s0009-2797(99)00053-8 |
| Popis: | Because of deficiencies in the present treatments for organophosphorus anticholinesterase poisoning, we are attempting to develop a catalytic scavenger that can be administered as prophylactic protection. Currently known enzymes are inadequate for this purpose because they have weak binding and slow turnover, so we are trying to make an appropriate enzyme by protein engineering techniques. One butyrylcholinesterase mutant, G117H, has the desired type of activity but reacts much too slowly. This communication describes an attempt to determine the reason for the slow reaction so that a more efficient enzyme might be designed. The results indicate that the mutation at residue 117 has resulted in a distortion of the transition state of the reaction of organophosphorus compounds with the active site serine. This information will be used to develop other mutants that avoid transition state stabilization sites. |
| Databáze: | OpenAIRE |
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