Production and antioxidant activity of intracellular polysaccharide by Hypsizigus marmoreus SK-01
| Autor: | Shanglong Gao, Suqian Wu, Liqin Wang, Long Hao, Xinyi Sun, Lifei Pang, Honghong Liu, Le Jia, Shouhua Jia, Mengshi Jia, Zhaogang Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Environmental Engineering
Antioxidant DPPH medicine.medical_treatment lcsh:Biotechnology Bioengineering Polysaccharide Central composite design Superoxide dismutase chemistry.chemical_compound Antioxidant activity In vitro lcsh:TP248.13-248.65 In vivo medicine Hypsizigus marmoreus SK-01 Food science Waste Management and Disposal EC50 chemistry.chemical_classification biology Superoxide Glutathione peroxidase Intracellular polysaccharide Biochemistry chemistry Catalase biology.protein |
| Zdroj: | BioResources, Vol 7, Iss 4, Pp 5879-5893 (2012) |
| ISSN: | 1930-2126 |
| Popis: | The extraction parameters of intracellular polysaccharide (IPS) from Hypsizigus marmoreus SK-01 mycelia were optimized, and the antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be: an ultrasonic treatment time of 618.07 s, an extraction temperature of 84.53 °C, a pH of 7.57, and an IPS yield of ca. 6.84%. The in vitro inhibition effects of IPS on hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 52.63 ± 3.19%, 68.21 ± 5.09%, and 63.43 ± 5.27%, respectively, and the EC50 values of IPS were 0.85 ± 0.05 g/L, 0.47 ± 0.03 g/L, and 0.62 ± 0.04 g/L, respectively. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in mice blood were 2.52 ± 0.02 U/mL, 453.19 ± 38.43 U/mL, and 118.59 ± 9.64 U/mL, respectively. The results provide a reference for large-scale extraction of IPS by H. marmoreus SK-01 in industrial fermentation, suggesting that IPS can be used as a potential antioxidant that enhances adaptive immune responses. |
| Databáze: | OpenAIRE |
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