Genetic diversity and hybrid formation in Central European club-mosses (Diphasiastrum, Lycopodiaceae) – New insights from cp microsatellites, two nuclear markers and AFLP
Autor: | Martin Schnittler, Radvilė Rimgailė-Voicik, Anja Klahr, Karsten Horn, Rico Kaufmann, Manuela Bog, H. Wilfried Bennert, Jörg Fuchs |
---|---|
Rok vydání: | 2019 |
Předmět: |
Genetic Markers
0106 biological sciences 0301 basic medicine Chloroplasts Bryophyta 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Diphasiastrum Genetics Lycopodiaceae Amplified Fragment Length Polymorphism Analysis Molecular Biology Alleles Phylogeny Ecology Evolution Behavior and Systematics Hybrid Cell Nucleus Principal Component Analysis Genetic diversity biology Reproduction Genetic Variation biology.organism_classification Introns Europe 030104 developmental biology Haplotypes Evolutionary biology Backcrossing Alternation of generations Hybridization Genetic Amplified fragment length polymorphism Lycopodiophyta Microsatellite Repeats |
Zdroj: | Molecular Phylogenetics and Evolution. 131:181-192 |
ISSN: | 1055-7903 |
Popis: | In Europe, the genus Diphasiastrum (Lycopodiophyta) forms a reticulate network of six diploid taxa, including three parent species (D. alpinum, D. complanatum and D. tristachyum) and three hybrids (D. × issleri, D. × oellgaardii and D. × zeilleri). It was not clear if the hybrids arose once or repeatedly, if they have reproductive competence and if backcrossing occurs. We addressed these questions by analysing 209 accessions for chloroplast microsatellites (cp), two nuclear markers (introns of the RPB and LFY genes) and AFLP. For D. complanatum we show a sexual life cycle with alternation of generations: the gametophytic DNA amount is half of that of the sporophyte. With the exception of a single accession all hybrids display one of the two parental cp haplotypes; their frequencies do not differ significantly from a 1:1 ratio. Genotypes of nuclear markers are species-specific, displaying 2/4/1 (RPB) and 1/8/1 alleles (LFY) for the three parents mentioned above; all hybrids have one allele from each parent. All three hybrid taxa apparently represent independent F1 crosses. Hybridisation occurs bidirectional; no evidence for recent backcrossing was found. Asexual reproduction via agamospory is at least rare, since AFLP showed all hybrid plants to be different. |
Databáze: | OpenAIRE |
Externí odkaz: |