Molecular relationships between U snRNP proteins as investigated by rabbit antisera and peptide mapping
| Autor: | Susanne Rothe, Rolf Reuter, Reinhard Lührmann |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Enzyme-Linked Immunosorbent Assay
Peptide Cross Reactions Autoantigens Peptide Mapping snRNP Core Proteins Epitope Epitopes Genetics Animals Humans snRNP Ribonucleoprotein chemistry.chemical_classification Antiserum Chymotrypsin SnRNP Core Proteins biology Immune Sera Ribonucleoproteins Small Nuclear Molecular biology Ribonucleoproteins Biochemistry chemistry biology.protein Rabbits Antibody Peptides |
| Zdroj: | Nucleic Acids Research. 15:4021-4034 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/15.10.4021 |
| Popis: | Each of the major U snRNP polypeptides from human cells was purified by electroelution from SDS-polyacrylamide gels. Rabbit antisera could be obtained against the individual proteins 70K, A, B', B and D, although rabbits failed to elicit antibodies against E, F and G. A strong structural homology was found between proteins B' and B, against which patients with connective tissue diseases produce predominantly anti-Sm autoantibodies. Thus, rabbit antisera against B' strongly crossreact with B and vice versa. Peptide patterns of the proteins B' and B obtained with chymotrypsin are identical with the exception of one fragment in each case. Polypeptide D, the third major Sm-antigenic protein, is structurally distinct from B' and B, as evidenced by the failure of anti-D antisera to crossreact with B' or B and vice versa, as well as by the different peptide patterns observed for proteins D and B' or B. The U1 specific polypeptide A and the U2 specific polypeptide B" share homologous regions, as indicated by the crossreactivity of anti-A antisera with protein B", and the occurrence of common fragments in the peptide patterns of the two proteins. Further homologies between other snRNP protein pairs were not detected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|