Delayed moderate hypothermia reduces calpain activity and breakdown of its substrate in experimental focal cerebral ischemia in rats
| Autor: | Martin Liebetrau, Helge K. Martens, Dorothe Burggraf, Gerhard F. Hamann, Mathias Pichler |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Telencephalon medicine.medical_specialty Central nervous system Ischemia Down-Regulation Functional Laterality Brain Ischemia Brain ischemia Western blot Hypothermia Induced Internal medicine medicine Animals Rats Wistar biology medicine.diagnostic_test Calpain Cerebrum General Neuroscience Infarction Middle Cerebral Artery Hypothermia medicine.disease Immunohistochemistry Rats Up-Regulation Enzyme Activation Disease Models Animal Endocrinology medicine.anatomical_structure Anesthesia biology.protein medicine.symptom Microtubule-Associated Proteins Intracellular Peptide Hydrolases |
| Zdroj: | Neuroscience Letters. 357:17-20 |
| ISSN: | 0304-3940 |
| Popis: | Calpains, intracellular proteases, are involved in various cerebral disorders. To determine the effect of moderate hypothermia on calpain activity, transient middle cerebral artery occlusion in rats was performed. For the reperfusion period normothermic temperature was compared to post-ischemic hypothermia (32 degrees C). Calpain expression was measured by Western blot analysis and immunohistochemistry. The loss of calpain substrate was determined by immunohistochemistry against the anti-microtubule-associated protein-2 (MAP-2). The increase of calpains in the ischemic as compared to the non-ischemic contralateral hemisphere and the loss of MAP-2 were reduced by hypothermia. These data indicate that calpain activity and calpain-induced proteolysis play an important role in the network of events following cerebral ischemia and can be reduced by hypothermia. Moderate hypothermia may be a useful tool to limit secondary injury induced by intracellular calpain degradation. |
| Databáze: | OpenAIRE |
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