Rutin inhibits proliferation, attenuates superoxide production and decreases adhesion and migration of human cancerous cells
| Autor: | Mohamed Mousslim, Mohamed Ben Sghaier, Alessandra Pagano, Youssef Ammari, Hervé Kovacic, José Luis |
|---|---|
| Přispěvatelé: | Laboratoire d'Ecologie Forestière, Institut National de Recherche en Génie Rural Eaux et Forêts (INRGREF), Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF)-Institution de la Recherche et de l'Enseignement Supérieur Agricoles [Tunis] (IRESA)-Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF)-Institution de la Recherche et de l'Enseignement Supérieur Agricoles [Tunis] (IRESA), Université de Carthage - University of Carthage, Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Rutin Flavonoid [SDV.CAN]Life Sciences [q-bio]/Cancer Extracellular matrix 03 medical and health sciences chemistry.chemical_compound HT29 Cells Superoxide production 0302 clinical medicine Cell Movement Superoxides Cell Adhesion Humans Lucigenin Migration Cell Proliferation Pharmacology chemistry.chemical_classification Dose-Response Relationship Drug Superoxide General Medicine Adhesion Molecular biology Colorectal cancer In vitro 3. Good health 030104 developmental biology chemistry Biochemistry A549 Cells 030220 oncology & carcinogenesis [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology Caco-2 Cells Lung cancer Reactive Oxygen Species |
| Zdroj: | Biomedicine and Pharmacotherapy Biomedicine and Pharmacotherapy, 2016, 84, pp.1972-1978. ⟨10.1016/j.biopha.2016.11.001⟩ Biomedicine and Pharmacotherapy, Elsevier Masson, 2016, 84, pp.1972-1978. ⟨10.1016/j.biopha.2016.11.001⟩ |
| ISSN: | 0753-3322 1950-6007 |
| DOI: | 10.1016/j.biopha.2016.11.001⟩ |
| Popis: | International audience; Lung and colorectal cancer are the principal causes of death in the world. Rutin, an active flavonoid compound, is known for possessing a wide range of biological activities. In this study, we examined the effect of rutin on the viability, superoxide anion production, adhesion and migration of human lung (A549) and colon (HT29 and Caco-2) cancer cell lines. In order to control the harmlessness of the tested concentrations of rutin, the viability of cancer cell lines was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. ROS generation was measured by lucigenin chemiluminescence detecting superoxide ions. To investigate the effect of rutin on the behavior of human lung and colon cancer cell lines, we performed adhesion assays, using various purified extracellular matrix (ECM) proteins. Finally, in vitro cell migration assays were explored using modified Boyden chambers. The viability of cancerous cells was inhibited by rutin. It also significantly attenuated the superoxide production in HT29 cells. In addition, rutin affected adhesion and migration of A549 and HT29 cell. These findings indicate that rutin, a natural molecule, might have potential as anticancer agent against lung and colorectal carcinogenesis. |
| Databáze: | OpenAIRE |
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